华北五角枫‘京2’插穗生根过程中内源激素变化

为建立高效的扦插技术体系,运用酶联免疫吸附测定法,测定了华北五角枫‘京2’扦插过程中插穗内生长素(IAA)、脱落酸(ABA)、赤霉素(GA)、玉米素(ZR)4种植物内源激素的含量,研究了华北五角枫‘京2’扦插生根的生理生化机理。结果表明:华北五角枫‘京2’扦插生根过程中插穗内源激素含量与生根密切相关,插穗根原基分化形成及生长发育在高浓度的内源IAA及低浓度的内源ABA作用下被促进,华北五角枫‘京2’插条不定根的形成在高浓度的内源GA作用下会被抑制;华北五角枫‘京2’扦插生根的不同时期,其内源ZR的作用不同,低浓度的内源ZR促进根原基分化形成,而较高浓度的内源ZR有利于根原基生长发育;4种内源激素的初始含量与生根率的相关性表现出不同,ABA、GA的初始含量与生根率呈负相关,而IAA、ZR呈正相关,其中IAA与生根率关系最密切。

Changes of Endogenous Hormones During Acer truncatum Bunge Cutting

In order to establish efficient cutting technique system, contents of 4 kinds of endogenous hormones (IAA、GA、ABA and ZR) in Acer truncatum Bunge cutting were determined with ELISA, and physiological and biochemical mechanism of rooting during cutting was studied. The results showed that contents of the endogenous hormones were closely related to rooting during cutting in Acer truncatum Bunge. Endogenous IAA at higher concentration level and endogenous ABA at lower level helped for formation and growth of root primordium in cuttings, and endogenous GA at higher level inhibited formation of adventitious root in cuttings. During different cutting periods, the functions of endogenous ZR were different, lower concentration promoted differentiation and formation of root primordium, while higher concentration benefited to growth and development of root primordium; initial contents of 4 endogenous hormones had different correlations with rooting rate, those of IAA and ZR were positively correlated, while those of ABA and GA were negative correlated, in which IAA was most closely with rooting rate.