| 侵染云南烟草的番茄环纹斑点病毒N基因的分子变异分析 |
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| 引用本文： | 卢训,丁铭,方琦,方敦煌,秦西云,张仲凯.侵染云南烟草的番茄环纹斑点病毒N基因的分子变异分析[J].植物病理学报,2012,42(2):195-201. |
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| 作者姓名： | 卢训 丁铭 方琦 方敦煌 秦西云 张仲凯 |
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| 作者单位： | 1云南省农业科学院生物技术与种质资源研究所 云南省农业生物技术重点实验室 农业部西南农业基因资源与种质创制重点实验室，昆明 650223； 2云南省烟草农业科学研究院， 玉溪653100 |
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| 基金项目： | 国家自然科学基金资助项目(30860163);中国烟草公司重点科技项目(110200902065);云南省烟草公司科技项目(2010YN19) |
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| 摘 要： | 应用电镜观察和ELISA检测从云南11个县（市）烟草种植区采集的烟草上检测到番茄环纹斑点病毒（Tomato zonate spot virus, TZSV）。根据TZSV N基因设计引物扩增得到了31个TZSV分离物N基因全序列。测序分析表明，31个TZSV N基因核苷酸序列与GenBank中报道的序列相似性在94.9%~98%之间，其推导的氨基酸序列同源性为98.2%~99.3%。构建系统进化树发现云南不同地区的TZSV分离物存在一定差异。氨基酸序列比较发现，文山分离物和西畴分离物具有2个该地区独有的氨基酸位点。
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| 关 键 词： | 番茄环纹斑点病毒 N基因 分子变异 |
Molecular variation of natural populations of Tomato zonate spot virus infecting flue-cured tobacco in Yunnan |
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| LU Xun
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DING Ming
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FANG Qi
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FANG Dun-huang
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QIN Xi-yun
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ZHANG Zhong-kai.Molecular variation of natural populations of Tomato zonate spot virus infecting flue-cured tobacco in Yunnan[J].Acta Phytopathologica Sinica,2012,42(2):195-201. |
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| Authors: | LU Xun DING Ming FANG Qi FANG Dun-huang QIN Xi-yun ZHANG Zhong-kai |
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| Institution: | 1Institute of Biotechnology and Germplasm Resources，Yunnan Academy of Agricultural Sciences, Yunnan Province Key Laboratory of Agricultural Biotechnology, Key Lab of the Southwestern Crop Gene Resources and Germplasm Innovation, Ministry of Agriculture, Kunming 650223, China; 2Yunnan Tobacco Research Institute, Yuxi 653100, China |
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| Abstract: | Using electron microscopy and ELISA,Tomato zonate spot virus(TZSV) isolates were detected from flue-cured tobacco in 11 counties and cities of Yunnan.With primers from conserved regions,the nucleocapsid(N) gene sequences of 31 naturally occurring TZSV isolates from flue-cured tobacco were amplified by RT-PCR.Sequence analysis showed a high level of sequence conservation(94.9%~98%) among the N genes of the tobacco isolates.However,cluster dendrogram showed distinct differences were present in Yunnan TZSV isolates from different area.Amino acid sequence analysis showed that two amino acid residues were considered to be area specific in Wenshan isolates and Xichou isolates. |
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| Keywords: | Tomato zonate spot virus nucleocapsid gene molecular variation |
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