乌拉尔甘草组培再生体系的研究

甘草是兼具生态和经济双重价值的重要植物资源,人工培育以野生变家种为主,尚未开展系统的育种工作。本研究以内蒙古杭锦旗的野生乌拉尔甘草为材料,研究了外植体、基本培养基、NAA、TDZ和蔗糖对丛生芽诱导的影响以及微繁体系的建立。结果表明,适合甘草丛生芽诱导的外植体为生长4~7 d的无菌苗子叶节,培养基为MS TDZ 0.1 mg/L NAA 0.1 mg/L 蔗糖20~30 g/L;将带1叶的茎段在改良MS NAA 0.1 mg/L 6-BA1.0~2.0 mg/L的培养基中培养,其增殖倍数可稳定达到25倍;在1/2MS 核黄素2.0 mg/L中生根,炼苗3 d移栽到蛭石中,成活率达96%以上。1个带腋芽茎段培养3个月可获得13 906棵生根试管苗,成活13 350株,繁殖效率高达85%。

Establishment of micropropagation system of Glycyrrhiza uralensis

Glycyrrhiza uralensis is one of the most important and widely used Chinese traditional medicinal plants.The domestication of wild plants is still the main procedure as no systematic breeding programs have been carried out.This study examined the effects of explants,culture media,NAA,TDZ and sugar on inducement of fasciculate buds and on the establishment of a micropropagation system of wild licorice from Hangjinqi,Inner Mongolia.Suitable explants for micropropagation were the cotyledonary nodes from 4 to 7 day old germ-free plants and the initial medium was MS TDZ 0.1 mg/L NAA 0.1 mg/L sugar 20-30 g/L.When the segment with one leaf was inoculated on the improved MS 6-BA 1.0-2.0 mg/L NAA 0.1 mg/L,the newly formed buds grew regularly and the proliferation efficiency was as high as 25 times.After 1-2 cm adventitious roots were produced on 1/2MS VB2 1.0-2.0 mg/L with a rooting percentage of 86%,the plantlets were transplanted to sterile vermiculite directly without any excision and were sprayed with 1/800 Carbendazim CIPAC.The survival rate was about 96%.Thus from only one segment,13 906 plantlets with roots were formed in 3 months of which 13 350 plants survived.The proliferation efficiency was higher than 85%.