产挥发性物质芽胞杆菌对枇杷炭疽病菌的抑制作用及其鉴定

采用二分隔特殊平板研究芽胞杆菌挥发性物质对枇杷炭疽病菌Colletotrichum acutatum的抑制作用。研究结果表明，筛选得到的8株芽胞杆菌菌株中，抑菌效果最好的菌株为FJA T-4748，抑菌率达90.06％；其次为FJAT-10011，抑菌率达54.99％。经16S rDNA序列鉴定结合菌落形态观察，确认这8株芽胞杆菌分别为解木糖赖氨酸芽胞杆菌 Lysinibacillus xylanilyticus、波茨坦短芽胞杆菌 Brevibacillus borstelensis、短短芽胞杆菌Brevibacillus brevis、土壤短芽胞杆菌 Brevibacillus agri、阿氏芽胞杆菌 Bacillus aryabhattai、美丽短芽胞杆菌Brevibacillus formosus及简单芽胞杆菌Bacillus simplex。本研究筛选的产挥发性物质芽胞杆菌菌株可为枇杷采后炭疽病的生物防治提供菌株参考。

Identification of Volatile Compounds Produced Bacillus spp .and Their Antimicrobial Effects on the Anthracnose Pathogen from Postharvest Loquat

The volatile compounds produced strains of Bacillus spp .were antagonistic to the loquat anthracnose pathogen （Colletotrichum acutatum） in two-compartment plastic plates .The results showed that the inhibition rates of FJAT-4748 and FJAT-10011 were 90.06% and 54.99% ,which were better than the other tested strains .Colony morphology and 16S rDNA sequence assays showed that the tested eight bacterial strains of Bacillus spp .were Lysinibacillus xylanilyticus、 Brevibacillus borstelensis , Brevibacillus brevis , Brevibacillus agri , Bacillus aryabhattai ,Brevibacillus formosus and Bacillus simplex ,respectively .Our results suggested that these volatile compounds produced Bacillus strains could be promising biocontrol strains for anthracnose in harvested loquat fruit .