| 解几丁质类芽胞杆菌几丁质酶活性及其次生代谢物的测定 |
| |
| 引用本文： | 郑梅霞,陈峥,刘波,刘程程,车建美,蓝江林,潘志针,朱育菁,唐建阳.解几丁质类芽胞杆菌几丁质酶活性及其次生代谢物的测定[J].福建农业学报,2014(5):492-497. |
| |
| 作者姓名： | 郑梅霞 陈峥 刘波 刘程程 车建美 蓝江林 潘志针 朱育菁 唐建阳 |
| |
| 作者单位： | [1]福建省农业科学院农业生物资源研究所,福建福州350003 [2]福建农林大学生命科学院,福建福州350002 |
| |
| 基金项目： | 科技部973前期项目（2011CB111607）;农业部948项目（2011-G25）;福建省财政专项--福建省农业科学院科技创新团队建设基金（STIFY03）;国家自然科学基金（31370059）;福建省农业现代化发展战略研究项目（ZDZX-1301）;科技部国际合作项目（2012DFA31120） |
| |
| 摘 要： | 采用几丁质酶培养基筛选出 FJAT-10038芽胞杆菌，测定其几丁质酶活力为11.04 U·mL^-1，说明FJA T-10038芽胞杆菌能够产几丁质酶。采用气相色谱质谱联用（GC/M S ）的方法分析FJA T-10038发酵液丙酮萃取的成分，初步鉴定出31种化合物。在鉴定出的化合物中，相对含量超过10％的有2种，分别是六氢化-吡咯环1，2-a ]-吡嗪-1，4-二酮和异丁醚，相对含量分别为44.17％和15.58％；匹配率在90％以上的成分有3种，分别是二叔丁基对甲酚、六氢化-吡咯环1，2-a]-吡嗪-1，4-二酮和六氢-3-苯甲基-吡咯并1，2-a]-吡嗪-1-4-二酮，匹配度分别是90％、97％和93％，相对含量分别是0.43％、44.17％和0.38％；另外还发现一些其他的活性物质。
|
| 关 键 词： | 几丁质酶 气相色谱质谱联用 解几丁质类芽胞杆菌 丙酮萃取 |
The Caracteristics of Citinase Ativity and Scondary Mtabolites in Paenibacillus Citinolyticus |
| |
| ZHENG Mei-xia,CHEN Zhen,LIU Bo,LIU Cheng-cheng,CHE Jian-mei,LAN Jiang-lin,PAN Zhi-zhen,ZHU Yu-jing,TANG Jian-yang.The Caracteristics of Citinase Ativity and Scondary Mtabolites in Paenibacillus Citinolyticus[J].Fujian Journal of Agricultural Sciences,2014(5):492-497. |
| |
| Authors: | ZHENG Mei-xia CHEN Zhen LIU Bo LIU Cheng-cheng CHE Jian-mei LAN Jiang-lin PAN Zhi-zhen ZHU Yu-jing TANG Jian-yang |
| |
| Institution: | 1. Agricultural Bioresource Research Institute ,Fujian Academy of Agricultural Sicences, Fuzhou, Fujian 350003, China; 2. College of Life Science, FujianAgriculture and Forestry University, Fuzhou, Fujian 350002, China) |
| |
| Abstract: | The chitinase activity of Paenibacillus chitinolyticus FJAT-10038 was characterized by filtering FJAT-10038 Bacillus using chitinase medium ,the chitinase activity of which reached at 11.044 U mL ^-1 .Furthermore , Acetone extracts of FJAT-10038 Bacillus were analyzed by GC-MS . Our results suggest that thirteen-one compounds are identified ,and the content of Pyrrolo 1 ,2-a] pyrazine-1 ,4-dione ,hexahydro- and Propane ,1-ethoxy-2-methyl-reached 44.1672% and 15.5814% .Meanwhile ,the match degree of Butylated Hydroxytoluene , Pyrrolo 1 , 2-a ] pyrazine-1 , 4-dione , hexahydro- and Pyrrolo 1 , 2-a ] pyrazine-1 , 4-dione , hexahydro-3-（phenylmethyl）-were 90% ;97% and 93% ,with a relative amount by 0.4305% ,44.1672% and 0.3842% .In addition ,some other active substances were also been characterized . |
| |
| Keywords: | chitinase GC/MS Penibacillus chitinolyticus acetone extract |
| 本文献已被 维普 等数据库收录！ |
|
