| 马铃薯品种遗传多样性的RAPD和AFLP标记分析 |
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| 引用本文： | 李凤云,盛万民,刘昭军,田国奎,李庆全,王立春.马铃薯品种遗传多样性的RAPD和AFLP标记分析[J].中国马铃薯,2007,21(5):266-271. |
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| 作者姓名： | 李凤云 盛万民 刘昭军 田国奎 李庆全 王立春 |
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| 作者单位： | 1. 黑龙江省农业科学院马铃薯研究所,黑龙江,克山,161606
2. 黑龙江省农业科学院生物技术研究中心,黑龙江,哈尔滨,150086 |
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| 基金项目： | 黑龙江省农业科学院科研计划项目 |
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| 摘 要： | 利用RAPD和AFLP分子标记技术对19份马铃薯品种进行了遗传多样性分析。试验表明:在RAPD分析中,19份马铃薯品种的遗传距离介于0.1707￣0.7222之间,平均值为0.3917;AFLP分析表明,19份材料的遗传距离介于0.2091￣0.7679之间,平均值为0.4811。两种方法均适于马铃薯品种遗传多样性分析,其中RAPD简便、快速、成本低,较适用于分析马铃薯品种亲缘关系,指导育种实践,而AFLP技术具有很高的分辨率,更适于进行马铃薯品种鉴定。
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| 关 键 词： | 马铃薯 RAPD AFLP 聚类分析 遗传多样性 |
| 文章编号： | 1672-3635（2007）05-0266-06 |
| 修稿时间： | 2007-05-20 |
Genetic Diversity of Potato Cultivars Revealed by RAPD and AFLP Markers |
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| Li Fengyun,Sheng Wanmin,Liu Zhaojun,Tian Guokui,Li Qingquan,Wang Lichun.Genetic Diversity of Potato Cultivars Revealed by RAPD and AFLP Markers[J].Chinese Potato,2007,21(5):266-271. |
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| Authors: | Li Fengyun Sheng Wanmin Liu Zhaojun Tian Guokui Li Qingquan Wang Lichun |
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| Institution: | 1. Potato Research Institute, Heilongjiang Academy of Agricultural Sciences, Keshan, Heilongjiang 161606, China; 2. Biology Research Center, Heilongjiang Academy of Agricultural Sciences, Harbin, Heilongjiang 150086, China |
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| Abstract: | The RAPD and AFLP were used to study the genetic diversity of 19 potato cultivars. In RAPD, the genetic distance of 19 potato cultivars was between 0.1707- 0.7222, with average value being 0.3917. AFLP analysis indicated that the genetic distance of 19 potato cultivars was between 0.2091-0.7679, with average value being 0.4811. The two methods are both suitable to analyze the genetic diversity of potato cultivars. However, RAPD is more preferred to analyze the sibship of potato cultivars due to its simple technique and low cost. This study also demonstrates that AFLP finger printing has higher resolution and the AFLP is more suitable to reveal the difference of potato cultivars. |
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| Keywords: | RAPD AFLP |
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