| 老年大鼠海马CA1区锥体细胞凋亡的形态学观察 |
| |
| 引用本文： | 刘学忠,崔旭,任建新,卞建春,刘宗平,王捍东,王宗元.老年大鼠海马CA1区锥体细胞凋亡的形态学观察[J].扬州大学学报(农业与生命科学版),2004,25(4):72-74. |
| |
| 作者姓名： | 刘学忠 崔旭 任建新 卞建春 刘宗平 王捍东 王宗元 |
| |
| 作者单位： | 1. 扬州大学,兽医学院,江苏,扬州,225009
2. 解放军总医院,老年医学研究所,北京,100853 |
| |
| 基金项目： | 国家重点基础研究发展规划项目(G2000057000); 扬州大学畜牧兽医学院科研基金项目(2003006) |
| |
| 摘 要： | 采用股动脉放血并双侧颈总动脉夹闭法制作全脑缺血再灌注模型，于术后3d处死动物取脑，以常规电镜程序制作标本，观察海马CA1区锥体细胞超微结构的改变；同时结合原位末端标记法检测，探讨全脑缺血再灌注后海马CA1区锥体细胞的病理损伤形式。结果表明：与假手术相比．缺血再灌注3d后海马CA1区出现了较多的凋亡样改变的锥体细胞，证明在脑缺血再灌注损伤中．细胞凋亡是神经元死亡的一种重要形式。
|
| 关 键 词： | 大鼠 脑缺血 海马 凋亡 形态学 |
| 文章编号： | 1671-4652(2004)04-0072-03 |
| 修稿时间： | 2003年10月13 |
Pathological Observation of Pyramidal Cells in CA1 Region of the Hippocampus After Reperfusion Following Forebrain Ischemia in Old Rats |
| |
| LIU Xue-zhong,CUI Xu,REN Jian-xin,BIAN Jian-chuen,LIU Zong-ping,WANG Han-dong,WANG Zong-yuan.Pathological Observation of Pyramidal Cells in CA1 Region of the Hippocampus After Reperfusion Following Forebrain Ischemia in Old Rats[J].Journal of Yangzhou University:Agricultural and Life Science Edition,2004,25(4):72-74. |
| |
| Authors: | LIU Xue-zhong CUI Xu REN Jian-xin BIAN Jian-chuen LIU Zong-ping WANG Han-dong WANG Zong-yuan |
| |
| Institution: | LIU Xue-zhong~1,CUI Xu~2,REN Jian-xin~1,BIAN Jian-chuen~1,LIU Zong-ping~1,WANG Han-dong~1,WANG Zong-yuan~1 |
| |
| Abstract: | In order to observe the pathologic injures of pyramidal cells of CA_1 region in the hippocampus 3 d after reperfusion following 10 min forebrain ischemia, bilateral carotid arteries occlusion model of old rats were used and the changes of ultrastructure of pyramidal cells of CA_1 region in the hippocampus were observed with electron microscope; meanwhile, we observed the changes of pyramidal cells in CA_1 region of the hippocampus through Tunel (Terminal-deoxynucleotidyl transferase-mediated dUTP-biotin nick and labeling) staining. The results showed: Compared with the sham-group,the ultrastructure of pyramidal cells of CA_1 region in the hippocampus 3 d after reperfusion following 10 min forebrain ischemia showed apoptosis-like changes.it has been tested that Apoptosis may be an important form in the course of cerebral ischemia-reperfusion(IR) injury. |
| |
| Keywords: | rat cerebral ischemia hippocampus apoptosis morphology |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
