丁香和连翘属间杂种胚的离体培养

丁香和连翘是我国东北，特别是哈尔滨的主要观赏灌木之一，为了提高其观赏利用价值，于１９９１年在黑龙江省森林植物园开展了丁香与连翘属间杂试验。以不同白丁香单侏为母本与东北连翘进行了远缘杂交。采用带种皮和不带种皮两种材料，取不同时期杂种胚进行离体胚培养。研究结果表明：不同白丁香单株与东北连翘杂交亲合力差异很大。以白丁香５号为最佳；以杂交授粉后５０ｄ左右取不带种皮胚进行幼胚离体培养效果最佳，此时幼胚容易成

ISOLATED CULTURE OF GENERIC HYBRID EMBRYO OF SYRINGA AND FORSYTHIA

Svringa and Forsythia are one of the major ornamental flower and shrub in northeastern China. especially in Harbin. In order to improve the ornamental and utilizable value. the intergeneric hybridization experiment between Syringa and Forsythia was made in Botanical Garden of Heilongjiang Province in 1991. Based on the distant crossing between single-plant of syringa oblata var. alba and Forythia mandshurica, the isolated embryo with epiderm or without epiderm at different developed stage were cultured respectively. The results showes that the cross-affinity between different single-plant of syringa oblata var. alba and Forsythia mandshurica are significant different and No. 5 syringa oblata var alba is the best. The best results were obtained through the isolated culture of hybrid embryo without epiderm which had developed 50 days after crossing. the hybrid embryo at this stage are easy to survive and the plantlets were successfully induced from the hybrid embryo.