| RP-HPLC测定烈香杜鹃叶总黄酮含量的研究 |
| |
| 引用本文： | 牛迎凤,邵赟,梅丽娟,陶燕铎.RP-HPLC测定烈香杜鹃叶总黄酮含量的研究[J].安徽农业科学,2009,37(24):11339-11340. |
| |
| 作者姓名： | 牛迎凤 邵赟 梅丽娟 陶燕铎 |
| |
| 作者单位： | 中国科学院西北高原生物研究所,青海西宁810001;中国科学院研究生院,北京,100049;中国科学院西北高原生物研究所,青海西宁,810001 |
| |
| 基金项目： | 国家科技支撑计划项目(2007BAI45B00)课题3 |
| |
| 摘 要： | 目的]建立烈香杜鹃叶总黄酮含量测定的RP—HPLC方法，并比较它们的含量。方法]烈香杜鹃叶用甲醇提取，反相高效液相色谱法分析药材中槲皮素、山奈酚、异鼠李素3种黄酮甙元，并计算出总黄酮含量。结果]采用Kromasil C18柱（4．6mm×250mm），以甲醇-0．4％磷酸（60：40，V／V）为流动相，可使3种黄酮成甙元达到基线分离，平均加样回收率98．74％和98．98％，相对标准偏差1．11％和1．00％。结论]RP—HPLC法可作为总黄酮的定量分析方法。
|
| 关 键 词： | RP-HPLC 烈香杜鹃叶 槲皮素 山奈酚 异鼠李素 |
RP-HPLC Determination of Flavonoids in Leaves of Rhododendron athopogonoide Maxim |
| |
| NIU Ying-feng et al.RP-HPLC Determination of Flavonoids in Leaves of Rhododendron athopogonoide Maxim[J].Journal of Anhui Agricultural Sciences,2009,37(24):11339-11340. |
| |
| Authors: | NIU Ying-feng |
| |
| Institution: | NIU Ying-feng et al(Northwest Institute of Plateau Biology,Chinese Academy of Sciences,Xining,Qinghai 810001) |
| |
| Abstract: | Objective]The research aimed to establish RP-HPLC method for the determination of flavonoids in leaves of Rhododendron athopogonoide Maxim and compare their contents.Method]Quercetin,kaempferol and isorhamntin in leaves of Rhododendron athopogonoide Maxim were extracted by methanol and analyzed by RP-HPLC,and then the content of flavonoids were calculated.Result]When the analysis was performed on a C18 column(4.6 mm×250 mm)with methanol-0.4% phosphoric acid(60∶40,V/V) as mobile phase,the quercetin,kaempferol and isorhamntin were separated well,and the average recoveroes were 98.74% and 98.98%,RSD were 1.11% and 1.00%.Conclusion] RP-HPLC could be used for quantitation determination of flavonoids. |
| |
| Keywords: | RP-HPLC |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
