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| 利用基因芯片检测水稻细菌性条斑病抗性相关基因 | |
| 引用本文： | 韩庆典,陈志伟,段远霖,兰涛,官华忠,周元昌,吴为人. 利用基因芯片检测水稻细菌性条斑病抗性相关基因[J]. 分子植物育种, 2008, 6(2): 239-244 |
| 作者姓名： | 韩庆典 陈志伟 段远霖 兰涛 官华忠 周元昌 吴为人 |
| 作者单位： | 福建农林大学作物遗传改良研究所,福州,350002;福建农林大学作物遗传改良研究所,福州,350002;福建农林大学作物遗传改良研究所,福州,350002;福建农林大学作物遗传改良研究所,福州,350002;福建农林大学作物遗传改良研究所,福州,350002;福建农林大学作物遗传改良研究所,福州,350002;福建农林大学作物遗传改良研究所,福州,350002 |
| 基金项目： | 国家自然科学基金 , 福建省自然科学基金 , 福建省青年科技人才创新基金 |
| 摘 要： | 细菌性条斑病(简称细条病)是水稻的主要病害之一.为了鉴定细条病的抗性基因,我们利用Affymetrix的基因芯片对抗病品种Acc8558和感病品种H359中受细条病菌侵染调控的基因进行高通量的检测.在两品种中共检测到956个差异表达基因,其中12个基因在两品种中一致上调,54个基因在两品种中一致下调,20个基因在两品种中表达变化方向相反.对差异表达基因的基因本性、代谢路径和启动子进行了分析.比较发现有30个差异表达基因的位置与已报道的控制水稻细条病的QTL吻合.本研究为水稻细条病抗性基因的克隆提供了线索,为揭示水稻细条病抗性的分子遗传机理奠定了基础. |
| 关 键 词： | 水稻 细菌性条斑病 差异表达基因 基因芯片 |
Detection of Genes Related to Resistance to Bacterial Leaf Streak in Rice Using Microarray |
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| Han Qingdian,Chen Zhiwei,Duan Yuanlin,Lan Tao,Guan Huazhong,Zhou Yuanchang,Wu Weiren. Detection of Genes Related to Resistance to Bacterial Leaf Streak in Rice Using Microarray[J]. Molecular Plant Breeding, 2008, 6(2): 239-244 | |
| Authors: | Han Qingdian Chen Zhiwei Duan Yuanlin Lan Tao Guan Huazhong Zhou Yuanchang Wu Weiren |
| Affiliation: | Han Qingdian Chen Zhiwei Duan Yuanlin Lan Tao Guan Huazhong Zhou Yuanchang Wu Weiren Crop Genetic Improvement Institute,Fujian Agriculture & Forestry University,Fuzhou,350002 |
| Abstract: | Bacterial leaf steak (BLS) is one of the most important diseases in rice. To identify BLS-resistant genes in rice, high-throughput detection of genes responding to the infection of BLS pathogen in a high-resistant variety Acc8558 and a high-susceptive variety H359 was performed using Affymetrix microrarray. A total of 956 differentially expressed genes (DEGs) were identified in the two varieties, of which 12 genes were consistently up-regulated and 54 consistently down-regulated in the two varieties, while ... |
| Keywords: | Rice Bacterial leaf streak Differentially expressed genes Microarray |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |