| 24份西番莲种质SSR分子标记分析 |
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| 引用本文： | 应东山,张如莲,王 明,王琴飞,高 玲,刘迪发,李莉萍.24份西番莲种质SSR分子标记分析[J].热带作物学报,2014(5):5-9. |
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| 作者姓名： | 应东山 张如莲 王 明 王琴飞 高 玲 刘迪发 李莉萍 |
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| 作者单位： | 中国热带农业科学院热带作物品种资源研究所农业部华南作物基因资源与种质创制重点开放实验室农业部热带作物种子种苗质量监督检验测试中心 海南儋州 571737);中国热带农业科学院热带作物品种资源研究所农业部华南作物基因资源与种质创制重点开放实验室农业部热带作物种子种苗质量监督检验测试中心 海南儋州 571737);中国热带农业科学院热带作物品种资源研究所农业部华南作物基因资源与种质创制重点开放实验室农业部热带作物种子种苗质量监督检验测试中心 海南儋州 571737);中国热带农业科学院热带作物品种资源研究所农业部华南作物基因资源与种质创制重点开放实验室农业部热带作物种子种苗质量监督检验测试中心 海南儋州 571737);中国热带农业科学院热带作物品种资源研究所农业部华南作物基因资源与种质创制重点开放实验室农业部热带作物种子种苗质量监督检验测试中心 海南儋州 571737);中国热带农业科学院热带作物品种资源研究所农业部华南作物基因资源与种质创制重点开放实验室农业部热带作物种子种苗质量监督检验测试中心 海南儋州 571737);中国热带农业科学院热带作物品种资源研究所农业部华南作物基因资源与种质创制重点开放实验室农业部热带作物种子种苗质量监督检验测试中心 海南儋州 571737) |
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| 基金项目： | 基金项目：中央级(公益性)科研院所基本科研业务费资助(No. PZS084)。 |
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| 摘 要： | 采用SSR标记对24份西番莲材料的遗传多样性进行研究，从45对引物组合中筛选出16对扩增带清晰、重复性好的引物组合。结果表明：16对SSR引物共产生68条扩增带，其中58条为多态性条带，多态性条带比率平均为85.3 %，24份材料的遗传相似系数(GS)的范围为0.09-1.0；UPGMA法聚类分析结果表明，24份西番莲种质以0.45为阈值可分为7大类，SSR标记丰富的多态性可在西番莲分子生物学领域中广泛应用。
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| 关 键 词： | 西番莲 SSR 种质资源 遗传多样性 |
SSR Analysis of Genetic Diversity of 24 Passion Fruit Germplasms |
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| YING Dongshan,ZHANG Rulian,WANG Ming,WANG Qinfei,GAO Ling,LIU Difa and LI Liping.SSR Analysis of Genetic Diversity of 24 Passion Fruit Germplasms[J].Chinese Journal of Tropical Crops,2014(5):5-9. |
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| Authors: | YING Dongshan ZHANG Rulian WANG Ming WANG Qinfei GAO Ling LIU Difa and LI Liping |
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| Institution: | Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China/Ministry of Agriculture Quality Control and Testing Centre for Seeds and Seedlings of Tropical Crops, Danzhou, Hainan 571737;Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China/Ministry of Agriculture Quality Control and Testing Centre for Seeds and Seedlings of Tropical Crops, Danzhou, Hainan 571737;Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China/Ministry of Agriculture Quality Control and Testing Centre for Seeds and Seedlings of Tropical Crops, Danzhou, Hainan 571737;Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China/Ministry of Agriculture Quality Control and Testing Centre for Seeds and Seedlings of Tropical Crops, Danzhou, Hainan 571737;Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China/Ministry of Agriculture Quality Control and Testing Centre for Seeds and Seedlings of Tropical Crops, Danzhou, Hainan 571737;Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China/Ministry of Agriculture Quality Control and Testing Centre for Seeds and Seedlings of Tropical Crops, Danzhou, Hainan 571737;Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences/Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China/Ministry of Agriculture Quality Control and Testing Centre for Seeds and Seedlings of Tropical Crops, Danzhou, Hainan 571737 |
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| Abstract: | 24 passion fruit accessions were studied for the analysis of genetic verity by SSR molecular marker. The results indicated that 24 accessions could be separated by 16 primer combinations which showed effective and reproductive selecting from 45 pairs of primer. The primers amplified 68 reproducible fragments with 58 bands were polymorphic in nature. Polymorphism ratio was 85.3%. GSC among the 24 accessions ranged from 0.09 to 1.0. When GSC 0.45 as the threshold, 24 passion fruit accessions were clustered into seven major groups by unweighted pair-group method with arithmetic averages. The results showed that SSR marker could be widely used in molecular genetic map construction and germplasm evaluation of passionfruit. |
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| Keywords: | passion fruit SSR germplasm resource genetic diversity |
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