| 燕山板栗叶片基因组AFLP反应体系建立 |
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| 引用本文： | 程丽莉,苏淑钗,秦岭,刘建立,尹伟伦. 燕山板栗叶片基因组AFLP反应体系建立[J]. 北京林业大学学报, 2006, 28(6): 35-39 |
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| 作者姓名： | 程丽莉 苏淑钗 秦岭 刘建立 尹伟伦 |
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| 作者单位： | 北京林业大学资源与环境学院,省部共建森林培育与保护教育部重点实验室;北京市农林科学院林业果树研究所;北京林业大学资源与环境学院,省部共建森林培育与保护教育部重点实验室;北京农学院植物科学技术系;中国林业科学研究院森林生态环境研究所 |
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| 摘 要： | 该文以燕山板栗嫩叶为材料，利用改良的CTAB法提取高质量的总DNA，通过优化了酶切连接、预扩增、选择性扩增等试验条件，得到了清晰的板栗AFLP指纹图谱.研究结果表明:① 应用经过改良的CTAB法可获得用于构建板栗AFLP体系的高质量DNA；②单独酶切和单独酶连体系比酶切酶连共体系效果更好，确定了板栗基因组DNA AFLP分子标记反应体系；③在所分析的9种引物组合中EAAC＋M-CAA、E-AAC＋M-CAT和E-AGT＋M-CAT 3个引物均获得较好的多态性.其中以E-AGT＋M-CAT引物对组合的扩增条带信号强度一致性好，条带分布均匀，得到了稳定、清晰、分辨率较高的指纹谱带.
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| 关 键 词： | 板栗 DNA提取 AFLP遗传标记 |
| 文章编号： | 1000-1522（2006）06-0035-05 |
| 收稿时间： | 2006-09-25 |
| 修稿时间： | 2006-09-25 |
Construction of AFLP reaction system in leaves of Castanea mollissima in the Yanshan Mountains |
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| CHENG Li-li,SU Shu-chai,QIN Ling,LIU Jian-li,YIN Wei-lun. Construction of AFLP reaction system in leaves of Castanea mollissima in the Yanshan Mountains[J]. Journal of Beijing Forestry University, 2006, 28(6): 35-39 |
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| Authors: | CHENG Li-li SU Shu-chai QIN Ling LIU Jian-li YIN Wei-lun |
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| Affiliation: | 1 Key Laboratory for Silviculture and Conservation, Ministry of Education, College of Natural Resources &; Environment, Beijing Forestry University,100083, P. R.China; 2 Institute of Forestry Pomology, Beijing Academy of Agricultural and Forestry Sciences, 100091, P. R. China; 3 Department of Plant Science, Beijing Agricultural College, 102206, P. R. China; 4 Institute of Forest Ecology，Environment and Protection，Chinese Academy of Forestry，Beijing，100091，P. R. China. |
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| Abstract: | High quality DNA required for construction of AFLP analysis system was isolated with an improved method of CTAB from young leaves of Castanea mollissima. A silver-staining AFLP reaction system was constructed for genetic analysis of C. mollissima by optimizing DNA restriction-ligase reaction, pre-amplification and selective amplification. Results showed that the system of restriction of DNA was better than restriction and link of DNA. The polymorphism bands were obtained from three primer pairs (E-AAC M-CAA, E-AAC M-CAT, E-AGT M-CAT), but E-AGT M-CAT could amplify more uniform and high sharp bands. Our study showed that the silver-staining AFLP was available for genetic diversification, variety identification, construction of molecular map, identification and classification. |
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| Keywords: | Castanea mollissima extraction of DNA AFLP genetic markers |
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