| 1个与卡瓦胡椒特异的SCAR标记的获得 |
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| 引用本文： | 施江,辛莉,谭琳,郑学勤.1个与卡瓦胡椒特异的SCAR标记的获得[J].热带作物学报,2007,28(2):50-53. |
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| 作者姓名： | 施江 辛莉 谭琳 郑学勤 |
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| 作者单位： | 1. 河南科技大学农学院,洛阳,471003
2. 热带作物生物技术国家重点实验室,海口,571101 |
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| 基金项目： | 国家科技攻关项目;河南科技大学校科研和教改项目 |
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| 摘 要： | 采用6份卡瓦胡椒材料、21份栽培胡椒和野生胡椒材料、1份不同属的草胡椒材料共计28份试验材料,在对它们进行了RAPD研究的基础上,进行了SCAR分子标记研究。设计1对卡瓦胡椒特异SCAR引物P8.1和P8.2,用这对特异引物对本次试验的28份材料进行PCR扩增,结果只有6份卡瓦胡椒材料扩增出了预期大小355bp的特异带,其它材料均无任何扩增。这说明引物P8.1和P8.2为卡瓦胡椒特异SCAR引物,这对卡瓦胡椒种质资源的鉴定有一定帮助。
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| 关 键 词： | 卡瓦胡椒 胡椒 |
| 修稿时间： | 2006-07-25 |
Amplification of a Kava Specific SCAR Molecular Marker |
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| Shi Jiang,Xin Li,Tan Lin,Zheng Xueqin.Amplification of a Kava Specific SCAR Molecular Marker[J].Chinese Journal of Tropical Crops,2007,28(2):50-53. |
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| Authors: | Shi Jiang Xin Li Tan Lin Zheng Xueqin |
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| Abstract: | In this experiment 6 accessions of kava(Piper methysticum),21 accessions of cultivated pepper and wild pepper,1 accession of Peperomia pellucida Kunth(28 accessions in all)were analysed with RAPD and then SCAR molecular marker.A pair of kava specific SCAR primers of P8.1 and P8.2 were designed and used to amplify 28 accessions by PCR.A specific band of an expected size of 355bp was amplified only from 6 kava accessions.This showed that P8.1 and P8.2 are two primers specific to kava and can be used for molecular identification of kava germplasm. |
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| Keywords: | RAPD SCAR |
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