| 转中国对虾抗菌肽基因水稻抗白叶枯病效应初析 |
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| 引用本文： | 王维,吴超,刘梅,刘旭日,胡国成,斯华敏,孙宗修,刘文真,付亚萍.转中国对虾抗菌肽基因水稻抗白叶枯病效应初析[J].中国水稻科学,2010,24(4):335-340. |
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| 作者姓名： | 王维 吴超 刘梅 刘旭日 胡国成 斯华敏 孙宗修 刘文真 付亚萍 |
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| 作者单位： | 1. 中国水稻研究所,水稻生物学国家重点实验室,浙江,杭州310006
2. 中国科学院,海洋研究所,海洋生物技术研发中心,山东,青岛,266071 |
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| 基金项目： | 国家转基因生物新品种培育科技重大专项资助项目 |
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| 摘 要： | 抗菌肽是一类具有抗菌活性的多肽类物质。利用农杆菌介导法将中国对虾抗菌肽基因np3和np5分别导入粳稻品种爱知旭中,PCR检测显示,T0代转np3基因和np5基因水稻植株的阳性率分别为36%和45%。RT-PCR检测T1代转基因水稻,结果表明抗菌肽基因在RNA水平上得到表达,而抗菌肽的表达对农艺性状未产生显著影响。用白叶枯病菌小种CR4分别对4个T1代转np3基因和np5基因株系进行接种,所有转基因株系均表现出比对照更高水平的抗性。为了验证抗菌肽基因的广谱抗性,进一步检测了np5转基因株系对3个菌株JS97-2、ZHE173和OS-225的抗性,发现中国对虾抗菌肽基因np5对白叶枯病菌可能具有较广谱的抗性。
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| 关 键 词： | 水稻 抗菌肽 白叶枯病 抗性 |
| 收稿时间： | 1900-01-01; |
Resistance of Antimicrobial Peptide Genes Transgenic Rice to Bacterial Blight |
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| WANG Wei,WU Chao,LIU Mei,LIU Xu-ri,HU Guo-cheng,SI Hua-min,SUN Zong-xiu,LIU Wen-zhen,FU Ya-ping.Resistance of Antimicrobial Peptide Genes Transgenic Rice to Bacterial Blight[J].Chinese Journal of Rice Science,2010,24(4):335-340. |
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| Authors: | WANG Wei WU Chao LIU Mei LIU Xu-ri HU Guo-cheng SI Hua-min SUN Zong-xiu LIU Wen-zhen FU Ya-ping |
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| Institution: | WANG Wei1,WU Chao1,LIU Mei2,LIU Xu-ri1,HU Guo-cheng1,SI Hua-min1,SUN Zong-xiu1,LIU Wen-zhen1,FU Ya-ping1,(1State Key Laboratory of Rice Biology,China National Rice Research Institute,Hangzhou 310006,China,2 R & D Center of Marine Biotechnology,Institute of Oceanology,Chinese Academy of Sciences,Qingdao 266071,China) |
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| Abstract: | Antimicrobial peptides (AMP) is a polypeptide with antimicrobial activity.The antimicrobial peptide genes np3 and np5 from Chinese shrimp were integrated into Oryza sativa L.subsp.japonica.cv.Aichi ashahi by Agrobacterium mediated transformation system.PCR analysis showed that the positive ratios of np3 and np5 were 36% and 45% in T0 generation,respectively.RT-PCR analysis showed that the antimicrobial peptide genes were expressed in T1 generation and there was no obvious difference in agronomic traits betw... |
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| Keywords: | rice antimicrobial peptides bacterial blight resistance |
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