| OsCtBP-A基因过量表达和RNAi沉默对水稻根系生长和抗性的影响 |
| |
| 引用本文： | 吴茂森,李磊,吴静,陈华民,何晨阳.OsCtBP-A基因过量表达和RNAi沉默对水稻根系生长和抗性的影响[J].中国农业科技导报,2010,12(1):106-110. |
| |
| 作者姓名： | 吴茂森 李磊 吴静 陈华民 何晨阳 |
| |
| 作者单位： | (中国农业科学院植物保护研究所, 植物病虫害生物学国家重点实验室, 北京 100193) |
| |
| 基金项目： | 国家转基因生物新品种培育重大专项(2009ZX08009-044B,2008ZX08001-002);;国家重点实验室自主研究课题专项(SKL2009SR03)资助 |
| |
| 摘 要： | 为了阐明己克隆的水稻C端结合蛋白家族基因OsCtBP-A的功能,构建了该基因的过量表达和RNAi沉默载体,通过农杆菌介导转化水稻品种日本晴愈伤组织,分别获得了45个过量表达和75个RNAi 沉默T0代转基因植株,并进行了PCR和GUS染色鉴定。T1代过量表达株OsCtBP-A基因表达量明显增加,苗期根系生长增加,对水稻白叶枯病抗性无明显变化；而基因沉默株OsCtBP-A基因表达量显著下降,苗期根系发育迟缓,抗旱性明显降低,抗病性减弱。表明OsCtBP-A基因的表达可能对水稻苗期根系发育、抗旱性和抗病性具有不同程度的调控作用。
|
| 关 键 词： | OsCtBP-A 过量表达 RNAi沉默 根系发育 抗性 |
| 收稿时间： | 2009-11-29 |
| 修稿时间： | 2009-12-14 |
Effects of Overexpression and RNAi-mediated Silence of OsCtBP-A Gene on Root Growth and Stress Resistance in Rice |
| |
| WU Mao-sen,LI Lei,WU Jing,CHEN Hua-min,HE Chen-yang.Effects of Overexpression and RNAi-mediated Silence of OsCtBP-A Gene on Root Growth and Stress Resistance in Rice[J].Journal of Agricultural Science and Technology,2010,12(1):106-110. |
| |
| Authors: | WU Mao-sen LI Lei WU Jing CHEN Hua-min HE Chen-yang |
| |
| Institution: | (State Key Laboratory for Biology of Plant Diseases and Insect Pests, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing 100193, China) |
| |
| Abstract: | In order to expound the functions of cloned C-terminal binding protein gene OsCtBP-A,its overexpression and RNAi constructs were constructed and transformed into calli of rice(cv.Nipponbare) via Agrobacterium-mediated transformation.Forty-five transgenic plants with overexpression and 75 with RNAi were generated and identified by PCR and GUS staining analysis.In T1 progeny of transgenic rice lines with overexpression,OsCtBP-A gene expression was remarkably increased,and root development was enhanced during ... |
| |
| Keywords: | OsCtBP-A overexpression RNAi-mediated silence root development stress resistance |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《中国农业科技导报》浏览原始摘要信息 |
| 点击此处可从《中国农业科技导报》下载免费的PDF全文 |
