外源C_4作物DNA导入小麦的研究

利用小麦自交后的花粉管通道,将C_4作物玉米或高粱的DNA导入小麦的种胚细胞,结果受体后代发生了广泛的变异,而且受体不同,所发生的变异不同。甘麦8号受体后代植株变矮,结实率低,分蘖多至5个,少至完全不分蘖;8347—2-4和8013—3品系后代中出现不育株;6873—20—3品系受体后代的籽粒由红色转变为白色而且稳定遗传;蓝粒麦受体后

Studies on DNA Transfer from C_4 Crop to Wheat

DNA extracted from C_4 crops (Chinese sorghum or maize) was transferred into the em-bryo of wheat through the pollen tube canal after self cross. This caused extensive variation inthe progenies of the acceptors, and the variations were different with different acceptors. Inthe progenies of Ganmai 8 acceptor, some plants became short--statured, with lower seed set-ting, and the number of tillers varied fronl zero to five. Some Plants of the progenies of8347--2--4 and 8013--3 became sterile, and the seed color of some seeds in the progeny of6873--20--3 changed from red to white and were bred true. Certain proportions of albino,white--green mosaic and green plants appeared in the D_1, D_2 and D_3 progenes of theBlue--seed acceptor. The albino plants died gradually before the 3--leaf stage, but the mosaicplants, although grew weakly could reach maturity and produce a few seed. Segregation con-tinued in the progenies of the mosaic plants as well as the green plants, but no segregation oc-curred in the progenies of the control. Results of PAGE scanning indicated that there were obvious differences in the types, ac-tivities or contents of isoperoxidase and soluble protein among the donors, acceptors and thethree types of D_2 leaf color variants at the 3--leaf stage. The Zymograms of the isoperoxidaseof the donor and the acceptor were quite different from each other, whereas those of D_2 vari-ants were intermediate between them but approaching the acceptors. Some specific bands ofthe donors appeared in the zymograms of the D_2 progenies and some bands showed indica-tion of heterozygosity. There were also differences among the albino, moszic and greenplants.