基因型及外源因子对小麦花药培养一步成苗的影响

研究了不同基因型及几种外源因子在小麦花药培养一步成苗中的效应。结果表明 ,花药培养一步成苗的愈伤组织诱导率、绿苗分化率、白苗分化率及绿苗产量存在基因型间的差异。高蔗糖浓度有利于获得高频率的花药愈伤组织 ,但同时又提高了花药培养中白化苗频率。动力精 (KT)对花药愈伤组织诱导率影响的差异不大 ,但对绿苗分化率、绿苗产量及绿苗 /白苗 (G/ W)均有显著影响。在试验浓度范围内 ,随 KT浓度的增大 ,绿苗产量提高 ,绿苗分化率明显增加 ,而白苗分化率下降 ,因而 G/ W值明显增大。说明 KT主要是通过提高绿苗分化率和降低白苗分化率 ,增大 G/ W而提高花培一步成苗效率的。在与 KT的配合使用中 ,2 ,4 - D比 NAA更有利于提高小麦花药培养中的愈伤组织诱导率和苗分化频率

Effect of genotype and extrafactor on forming seedling through one-step culture in anther culture of wheat

Effect of different genotype and extrafactor on forming seedling through one step culture in anther culture of wheat is studied.The following results are acquired.Induction frequency of pollen callua,calli giving albino plantlets and calli giving green plantlets have difference between genotype in forming seedling through one step culture.High concentration of sucrose (g/L) can raise the induction frequency of pollen callus. But at the same time,it also increase the calli giving albino plantlets.KT has not much effect on induction fuequency of pollen callus.However it has remarkable effect on calli giving albino plantlets,calli giving green plantlets and G/W.Within experimental concentration,with the increase of KT concentration,the number of the green plantlets and calli giving green plantlets will increase while the number of calli giving albino plantlets will decrease.So the value of G/W has remarkably increased.It indicates that KT increases the efficiency of forming seedling through one step culture by decreasing calli giving albino plantlets and increasing calli giving green plantlets.Combined with KT,2,4 D has higher induction frequency of pollen callus and ratio of plantlets differentiation than NAA in anther culture.