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韦塔桉叶片脂肪酸的提取及成分的GC-MS分析
引用本文:杨巧丽,谢耀坚,谭晓风,张婧,吴志华.韦塔桉叶片脂肪酸的提取及成分的GC-MS分析[J].桉树科技,2012(3):29-33.
作者姓名:杨巧丽  谢耀坚  谭晓风  张婧  吴志华
作者单位:[1]国家林业局桉树研发中心,广东湛江524000 [2]中南林业科技大学经济林育种与栽培国家林业局重点实验室,湖南长沙410000
基金项目:广东省自然科学基金项目(9145624536---400003)、湛江市财政资金科技专项竞争性分配项目(20102030244)和林业公益性行业科研专项(201104003)
摘    要:以丙酮为提取溶剂,物料粒度为100~200目,料液比1:15,浸泡8 h,萃取时间为1.5 h,在超声功率为80 khz,30℃时对韦塔桉桉叶油进行提取,并用GC-MS对其成分进行分析。结果得到韦塔桉桉叶油的提取率为19.04%,经GC-MS分析得到韦塔桉桉叶油含有10种脂肪酸,其中亚油酸和亚麻酸为不饱和脂肪酸。桉叶油中棕榈酸、亚油酸、亚麻酸及硬脂酸的绝对含量分别为0.82%、0.86%、0.15%、0.33%,占提取粗脂总量的2.16%。

关 键 词:韦塔桉  脂肪酸  提取  GC-MS

Extraction and GC- MS Analyses of Fatty Acids from Eucalyptus wetarensis Leaf Oils
YANG Qiao-li,XIE Yao-jian,TAN Xiao-feng,ZHANG Jing,WU Zhi-hua.Extraction and GC- MS Analyses of Fatty Acids from Eucalyptus wetarensis Leaf Oils[J].Eucalypt Science & Technology,2012(3):29-33.
Authors:YANG Qiao-li  XIE Yao-jian  TAN Xiao-feng  ZHANG Jing  WU Zhi-hua
Institution:YANG Qiao - li, XIE Yao - jian, TAN Xiao - feng, ZHANG Jing, WU Zhi - hua ( 1. China Eucalypt Research Centre, Zhanjiang 524022, Guangdong, China ; 2. Key Laboratory of Non- wood Forest Products of State Forestry Administration, Central University of Forestry and Teclmology , Changsha 410004, Hunan, China)
Abstract:Extraction of Eucalyptus wetarensis leaf oil was carried out with acetone as solvent,material particle size of 100~200 microns,solvent volume of 150 ml,immersion time of 8 h and extracting time of 1.5 h,in ultrasonic extractor of 80 khz at a temperature of 30℃.The content and components of fatty acids were analyzed by GC-MS.The results showed that the oil extraction rate was 19.04% by dry weight of leaf,and the oil included 10 types of fatty acids including two unsaturated fatty acid: linoleic acid and linolenic acid.The absolute contents of hexadecanoic acid,linoleic acid,linolenic acid and octadecanoic acid in Eucalyptus oils were: 0.82%,0.86%,0.15% and 0.33% respectively,and together fatty acids accounted for 2.16% by weight of the leaf oil.
Keywords:Eucalyptus wetarensis  fatty acid  extraction  GC-MS  
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