Assessment of EST- and genomic microsatellite markers for variety discrimination and genetic diversity studies in wheat |
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Authors: | Fiona Leigh Vince Lea John Law Petra Wolters Wayne Powell Paolo Donini |
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Institution: | (1) NIAB, Huntingdon Road, Cambridge, CB3 0LE, U.K;(2) DuPont Agricultural Biotechnology, Delaware Technology Park, Suite 200, 1 Innovation Way, Newark, DE, 19714, U.S.A;(3) Present address: Scottish Crops Research Institute, Invergowrie, Dundee, DD2 5DA, U.K. |
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Abstract: | It is likely that in the near future sequence information from sequencing programmes and EST libraries will generate an abundance
of genic microsatellite markers. This study is focused on the assessment of their likely impact and performance vis-à-vis
their genomic counterparts. Microsatellites from two sources were used to assess the genetic diversity in 56 old and new varieties
of bread wheat on the UK Recommended List. A set of 12 microsatellite markers generated from genomic libraries and 20 expressed
sequence tag (EST)-derived microsatellites were used in the study, and the performance of both marker sets assessed. The EST-derived
or genic microsatellites delivered fingerprints of superior quality, amplifying clear products with few stutter bands. Diversity
levels as revealed bygenic microsatellites are similar to the few published results. The PIC values for the genic markers
were generally lower than those calculated for the genomic microsatellites, though advantages of both marker classes for variety
identification applications are discussed.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | EST-SSR genic microsatellites genomic microsatellites molecular markers variety identification wheat |
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