防风花粉活力测定方法的比较及柱头可授性的研究

研究并确定防风花粉生活力及柱头可授性的快速有效测定方法。使用I2-KI、TCC、亚甲基蓝及无机盐培养法测定花粉活力。用联苯胺-过氧化氢法测定柱头可授性。亚甲基蓝染色法最适于检测花粉活力,结果与无机盐观测法符合度高。防风刚开花时花粉活力不高,开花后第三天花粉活力最高,可达96%,第五天花粉活力开始下降,且下降幅度较大。防风开花2d后柱头分泌黏液,第3d分泌量最大,但此时柱头不具有可授性。待花药脱落后,柱头可授性逐渐增强,第6-8d达到最大值。亚甲基蓝染色法简便、快捷,适于防风花粉活力的观测。花药脱落后第6-8d柱头可授性最强。

Research on the Determining Methods of the Pollen Viability and Stigmatic Receptivity of Saposhnikovia divaricata

To determined the rapid and efficient method for the determination of pollen viability of Saposhnikovia divari- cata and the method benzidine -hydrogen peroxide were used in the stigmatic receptivity. The methods, 12 - KI, TTC, methylene blue and, were used in the determination of the pollen viability. Its stigma receptivi- ty was estimated by the benzidine - hydrogen peroxide. Result Methylene blue staining method is fit to the detection for the pollen viability, and the resule accord with inorganic salts culture medium. The pollen via- bility of Saposhnikovia divaricata was lower in the early days of the blooming, the pollen viability went up to 96% at the third day after flower opening,and began to decline fastly at the 5th day after flowering. Stigmas began to secrete mucus 2 days after flowering, and it have maximum secretion at the third day after flower- ing, but at this time the stigmas have no receptivity. After the anthers dropping, receptivity gradually strengthened ,and it have maximum receptivity at 6 -8days after anthers dropping. Methylene blue method stained quickly, and it fit to the detection for the pollen viability and it have maximum receptivity at 6 - 8days after anthers dropping.