| Abstract: | Objective] The aim of this study was to provide a method for solving the problems in preparing BAC vector with High-copy plasmid pUC119-Bluelox BAG. Method] With 8electing a proper single restriction site, sequences of a single copy BAC vector plasmid were inserted into proper site of High-copy plasmid pUC119 vector. Result] The gene sequence of BAG vector lost control function of single copy number in new plasmid pUC119-BAC and was copied through High-copy form. The gene sequence of BAC vector basic function was completely cutted off through single enzyme digestion and the control function of single copy could be recovered by auto-connection. Conclusion ] The High-copy pUC119-BAC plasmid was used to copy and amplify high copy of basic function gene sequence in BAG vector, besides thai it could be used to construct transfer vector of molecular cloned recombinant virus or BAC library. |
