Immunodiagnosis of virulent strains of Aeromonas hydrophila associated with epizootic ulcerative syndrome (EUS) using a monoclonal antibody |
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Authors: | G A CARTWRIGHT D CHEN P J HANNA N GUDKOVS K TAJIMA |
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Institution: | Department of Biological Sciences, Deakin University, Victoria, Australia;Fish Health Reference Laboratory, Australian Animal Health Laboratory, CSIRO, Victoria, Australia;Department of Fisheries, Hokkaido University, Hakodate, Japan |
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Abstract: | Abstract. A virulent strain of Aeromonas hydrophila associated with epizootic ulcerative syndrome (EUS) was used to produce monoclonal antibodies that identified virulent strains of A. hydrophila. Antibodies from a clone, designated as F26P5C8, were found to identify the A. hydrophila serotype I isolates associated with EUS fish, and which were found to be virulent after subsequent inoculation studies. Immunodiagnosis of a large number of A. hydrophila from Australia and Japan showed some additional isolates to be identified by F26P5C8, but the status of their virulence is presently unknown. |
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