基于线粒体COI基因序列的挂墩稻弄蝶分类地位研究

对稻弄蝶属Parnara4种20个样本的线粒体COI基因序列(1380 bp)的特征、遗传距离进行了分析,并以2种谷弄蝶Pelopidas为外群,采用最大简约法(MP)和贝叶斯法(BI)构建系统树.结果表明:种间最小序列差异为3.3%,种内个体间最大序列差异为0.9%;系统树显示挂墩稻弄蝶Parnara batta与直纹稻弄蝶Parnara guttata是2个明显不同的分支;其种间的平均遗传距离为3.5%.综合地理分布与形态差异,挂墩稻弄蝶应该是一个独立的种.

Taxonomic Status of Parnara batta Evans (Lepidoptera:Hesperiidae) Inferred from Mitochondrial COI Gene Sequences

The characterization and genetic distance of mitochondrial COI gene sequence (1 380 bp) from 20 individuals covering 4 species were analyzed in the skipper genus Parnara.Phylogenetic trees were reconstructed with maximum parsimony (MP) and Bayesian inference (BI) using two Pelopidas species as outgroups.The results indicated that the smallest sequence divergence among species was 3.3%,whereas the largest instraspecific genetic distance was 0.9%.The tree showed that Parnara batta was quite different from Parnara guttata and strongly supported,and their average genetic distance was 3.5%.The molecular analysis and their sympatric distribution,together with the differences of morphological characteristics,suggested that Parnara batta is a distinct species.