| 传染性法氏囊病病毒超强毒HZ株和XN株VP2基因的克隆和序列比较 |
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| 引用本文： | 许信刚,王笑梅.传染性法氏囊病病毒超强毒HZ株和XN株VP2基因的克隆和序列比较[J].动物医学进展,2000,21(1):39-42. |
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| 作者姓名： | 许信刚 王笑梅 |
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| 作者单位： | 西北农林科技大学动物科学与动物医学学院!陕西杨陵712100，西北农林科技大学动物科学与动物医学学院!陕西杨陵712100，中国农业科学院哈尔滨兽医研究所，中国农业科学院哈尔滨兽医研究所，西北农林科技大学动物科学与动物医学学院!陕西杨陵712100，西北农林科技大学动物科学与动物 |
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| 基金项目： | 陕西省杨凌示范区专项基金 |
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| 摘 要： | 根据已发表的５２／７０株ＩＢＤＶ基因组序列，设计并合成了一对特异扩增ＩＢＤＶ ＶＰ２基因的引物。以陕西地区分离的ＩＢＤＶ野毒ＸＮ株，ＨＺ株为材料，以其基因组为模板利用ＲＴ－ＰＣＲ技术扩增出了１．５ｋｂ的ｃＤＮＡ产物，将ＶＰ２基因克隆于ＰＵＣ１１９质粒上，得到重组ＰＵＣ１１９质粒。
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| 关 键 词： | 传染性法氏囊病 序列分析 病毒 基因克隆 vvIBDV |
Cloning And Sequence Comparison of the VP2 Gene of Very Virulent Infectious Bursal Disease Virus Strains HZ and XN |
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| XU Xin gang ,LI Jian qiang ,WANG Xiao mei ,TONG Guang zhi ,LIANG Rong ,ZHANG Yao xiang ,XIN Fu shan.Cloning And Sequence Comparison of the VP2 Gene of Very Virulent Infectious Bursal Disease Virus Strains HZ and XN[J].Progress In Veterinary Medicine,2000,21(1):39-42. |
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| Authors: | XU Xin gang LI Jian qiang WANG Xiao mei TONG Guang zhi LIANG Rong ZHANG Yao xiang XIN Fu shan |
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| Institution: | XU Xin gang 1,LI Jian qiang 1,WANG Xiao mei 2,TONG Guang zhi 2,LIANG Rong 1,ZHANG Yao xiang 1,XIN Fu shan 1 |
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| Abstract: | According to the published sequence of IBDV strain 52/70, a pair of primers that can amplify the cDNA of protective antigen VP2 gene was designed and synthesized. By RT PCR, DNA fragments about 1.5 kb were obtained from IBDV strains HZ and XN. Then the VP2 cDNA was cloned into PUC119 at SmaI site. The nucleotide sequence of the expected VP2 gene was determined by Sanger's DNA sequencing method, and then the amino acid sequence was deduced. Both the nucleotide sequences and amino acid sequences were compared with five published sequences of VP2 gene of IBDV strains. It was shown that IBDV strains HZ,XN were most closely related to the very virulent IBDV strains UK661 and OKYM but different from other IBDV serotype I strains. |
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| Keywords: | Infectious bursal disease virus vvIBDV VP2 Cloning Sequence analyses |
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