云南猪住肉孢子虫的动物感染试验

 用大理、昆明、文山等地收集到的含猪住肉孢子虫包囊的生猪肉分别喂给实验猴、猫、犬,感染后第11天、第7天和第10天在猴、猫和犬的粪便中出现孢子囊。3种孢子囊的平均大小分别为13.50μm×10.25μm,11.75μm×9.25μm,13.25μm×10.75μm。从实验猴、猫、犬的粪便中分离收集孢子囊,分别接种给3头断奶仔猪。75天后剖检,在猪的心肌和骨骼肌中发现各种住肉孢子虫的包囊。包囊呈梭状、椭圆形或长椭圆形,并分隔成许多隔室,室中充满新月形缓殖子和卵圆形的母细胞,缓殖子的平均大小分别为10.86μm×5.24μm,10.04μm×4.84μm,11.14μm×5.74μm。结果表明云南有3种猪住肉孢子虫,即猪人住肉孢子虫,猪猫住肉孢子虫和米氏住肉孢子虫。

Infecitve Test of Yunnan Swine Sarcosporidium in Animals

The crude pork containing cysts of swine sarcosporidium collected from Xiaguan,Kunming and Winshan areas were respectively fed the experimental monkeys,cats and dogs.At 11 days,7 days,and 10 days after infection,the sporocysts were appeared in faeces of monkey,cat and dog.The sporocysts sizes of the three animals averaged 13.50μm×10.25μm,11.79μm×9.25μm,and 13.25μm×10.75μm.The colleted sporocysts were respectively inoculated three weanling pigs.75 days later,various cysts of sarcosporidium were found in myocardium and skeletal muscles.The cysts were spindle-shaped,elongated or elliptic,and were divided into many compartments.Which were full of crescent bradyzoites and ovate metrocytes.The average sizes of the three kinds of bradyzoites were 10.86μm×5.24μm,11.04μm×4.68μm,and 11.11μm×5.74μm.The results showed that there are three species namely S.suihominis,S.porcifelis and S.miescheriana in Yunnan.