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甘蓝型油菜三体、双三体和双四体的形态和细胞学特征
引用本文:华玉伟,完颜瑞红,徐晶,李再云.甘蓝型油菜三体、双三体和双四体的形态和细胞学特征[J].作物学报,2006,32(5):785-786.
作者姓名:华玉伟  完颜瑞红  徐晶  李再云
作者单位:华中农业大学作物遗传改良国家重点实验室/国家油菜改良(武汉)分中心,湖北武汉430070
基金项目:教育部跨世纪优秀人才培养计划;湖北省杰出青年科学基金;中国科学院资助项目
摘    要:从甘蓝型油菜品种“Oro”×诸葛菜杂交后的一个混倍体杂种(2n=19~37)的小孢子再生植株中鉴定出一株2n=42的植株,此混倍体不含有诸葛菜染色体,故2n=42植株的细胞内没有诸葛菜染色体。用其花粉授予“Oro”后获得2n=39、40的植株。2n=39、40、42植株PMCs内染色体的主要配对方式分别为19Ⅱ+1Ⅰ、19Ⅱ+2Ⅰ和19Ⅱ+2Ⅱ,后期Ⅰ的分离方式分别为20∶19、21∶19和21∶21。故2n=39、40、42为甘蓝型油菜的三体、双三体、双四体。最后讨论了这些种内非整倍体材料在油菜遗传研究中的意义。

关 键 词:甘蓝型油菜  三体  双三体  双四体  基因组原位杂交
收稿时间:2005-04-22
修稿时间:2005-04-222005-09-22

Morphological and Cytological Characterizations of Trisomics, Double Trisomics and Double Tetrasomics in Brassica napus
HUA Yu-Wei,WAN-YAN Rui-Hong,XU Jing,LI Zai-Yun.Morphological and Cytological Characterizations of Trisomics, Double Trisomics and Double Tetrasomics in Brassica napus[J].Acta Agronomica Sinica,2006,32(5):785-786.
Authors:HUA Yu-Wei  WAN-YAN Rui-Hong  XU Jing  LI Zai-Yun
Institution:National Key Laboratory of Crop Genetic Improvement, National Center of Oil Crop Improvement (Wuhan),Wuhan 430070, Hubei,China
Abstract:One plant (2n=42) was identified among the microspore—derived plants from one Brassica napus cv. Oro × Orychophragmus violaceus hybrid (2n=19~37), in which no O. violaceus chromosomes were detected, so the plant has only B. napus chromosomes. After Oro was pollinated with the pollen grains from the plant, progeny plants (2n=39, 40) were produced. Meiotic pairing configurations of the plants (2n=39, 40, 42) were mainly 19Ⅱ+1Ⅰ, 19Ⅱ+2Ⅰ and 21Ⅱ, and segregations at anaphase Ⅰ were 20∶19, 21∶19 and 21∶21, respectively. Thus the plants (2n=39, 40, 42) were trisomics, double trisomics and double tetrasomics of Brassica napus, respectively. Finally implications of these new aneuploids in rapeseed genetic researches were discussed.
Keywords:Brassica napus  Trisomies  Double trisomies  Double tetrasomies  Genomie in situ hybridization
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