SD大鼠热应激模型的制作及评价

为了对热应激SD大鼠模型的制作及生理生化指标进行检测。将20只SD大鼠随机分成两组，一组为对照组，二组为模型组，模型组在霉菌培养箱内以40℃高温、50%相对湿度热攻击2 h，使热应激发生，对照组正常饲养，2 h后观察大鼠行为变化、死亡个体剖检、眼底静脉丛采血、检测各种理化指标和肝脏Hsp70表达量。结果表明：与对照组相比，模型组的pH升高，差异不显著(P＞0.05)，Na⁺、K⁺浓度降低，其中K⁺达到差异极显著(P＜0.01)；血浆蛋白TP、ALB、HB浓度下降，达到差异极显著(P＜0.01)；血清酶ALT、GOT、ALP、LDH、CK活性均表现为升高，达到差异显著或极显著（P＜0.05或(P＜0.01)；SOD浓度降低，差异极显著(P＜0.01)，MDA浓度升高，差异极显著(P＜0.01)；肝脏Hsp70相对表达量升高，差异极显著(P＜0.01)。说明模型组符合热应激指征，SD大鼠热应激模型制作成功。

Making and Evaluation of SD Rat Heat Stress Model

For the establish of SD rats heat stress model and the testing of physical and chemical indexes. 20 SD rats were randomly divided into two groups, 1 group was the control group, 2 group was the model group, 2 group were heat attacked for 2 h in mould incubator with the temperature 40℃and relative humidity 50% , make heat stress occurred, the control group keep normal breeding, after two hours, observed behavior change, checked death individual, plexued blood by retinal vein , detected the physiological and biochemical indexes，detected the liver Hsp70 expression. The results showed that, compared with the control group, the concentration of K⁺ and Na⁺ were decreased, K⁺ reached extremely significant difference (P＜0.01); the concentration of ALB, HB and TP were decreased, reached extremely significant difference (P＜0.01); the activity of ALT, GOT, ALP, LDH, CK were increased, reached difference or significant difference（P＜0.05 or P＜0.01), the concentration of SOD was decreased, reached extremely significant difference (P＜0.01), the concentration of MDA was increased, reached extremely significant difference(P＜0.01);the expression of Hsp70 was increased, reached extremely significant difference (P＜0.01). The Model group was in accordance with heat stress indications, SD rats heat stress model was successfully established.