| 木聚糖酶的酶学性质研究 |
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| 引用本文： | 杨会涛,陈代文,田刚,余冰.木聚糖酶的酶学性质研究[J].中国畜牧杂志,2007,43(21):57-60. |
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| 作者姓名： | 杨会涛 陈代文 田刚 余冰 |
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| 作者单位： | 四川农业大学动物营养研究所,四川省动物营养与饲料工程重点实验室,四川雅安,625014 |
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| 摘 要： | 采用DNS法对木聚糖酶的一些性质进行了研究。结果表明:该酶的最适反应pH为6.0,在pH5～10之间保温不同时间后可保持较高的活性;最适反应温度50℃,液态下低于60℃热稳定性较好。在50℃、pH6.0下,以燕麦木聚糖为底物时,该木聚糖酶的米氏常数Km=6.49mg/mL,最大反应速度Vmax=21.05μg/(mL·min)。用不同底物测定酶活,以燕麦木聚糖为底物的酶活最高。酶在动物胃中活性较低,而在肠道中活性较高。
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| 关 键 词： | DNS 酶学性质 稳定性 食糜 木聚糖酶 |
| 文章编号： | 0258-7033（2007）21-0057-04 |
| 修稿时间： | 2006-05-25 |
Study on the Properties of Xylanase |
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| YANG Hui-tao,CHEN Dai-wen,TIAN Gang,YU Bing.Study on the Properties of Xylanase[J].Chinese Journal of Animal Science,2007,43(21):57-60. |
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| Authors: | YANG Hui-tao CHEN Dai-wen TIAN Gang YU Bing |
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| Abstract: | Properties of xylanases were studied by the method of DNS.The results showed that the optimal temperature and optimal pH of the xylanase was 50 ℃ and 6,respectively.Xylanase activity was stable between pH5 and pH10,and the themal stability of the enzyme was good in lipid below 60 ℃.The Km values and Vmax of xylanase for xylan from oat spelts under 50 ℃,pH6 were 6.49 mg/mL and 21.05 μg/(mL·min),respectively.The xylanase had the highest activity when xylan from oat spelts was used as the substrate.The enzyme activity was inhibited in stomach and restored in small intestine. |
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| Keywords: | DNS |
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