| 玉米自交系外源DNA导入后性状变异的初步探讨 |
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| 引用本文： | 周柱华, 张凤云, 徐立华, 邱登林, 邢燕菊, 阴卫军.玉米自交系外源DNA导入后性状变异的初步探讨[J].玉米科学,2006,14(2):006-009. |
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| 作者姓名： | 周柱华 张凤云 徐立华 邱登林 邢燕菊 阴卫军 |
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| 作者单位： | 山东省农业科学院原子能农业应用研究所,济南,250100 |
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| 摘 要： | 采用花粉管通道技术将大刍草的DNA导入玉米自交系黄早四中,连续3年对其后代的部分性状进行了调查分析,对性状的变异和转化进行了探讨。试验结果表明:导入大刍草DNA后,会降低黄早四的出苗率,使出苗时间延长,同时会使植株的株高、穗位高降低,并对果穗性状产生影响。处理后株系性状的变异范围均大于对照,证明导入大刍草的DNA会使自交系性状的变化范围增大,类型增多,扩大了育种的选择范围,增加了筛选机会。
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| 关 键 词： | 玉米自交系 大刍草 DNA 性状变异 生物技术 花粉管通道 |
| 文章编号： | 1005-0906（2006）02-0006-04 |
| 收稿时间： | 2005-04-13 |
| 修稿时间： | 5/8/2005 12:00:00 AM |
The Preliminary Study on the Variation of Maize Self-bred Line Characters by Exogenous DNA Transformation |
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| ZHOU Zhu-hu,ZHANG Feng-yun,XU Li-hu,QIU Deng-lin,XING Yan-ju,YIN Wei-jun.The Preliminary Study on the Variation of Maize Self-bred Line Characters by Exogenous DNA Transformation[J].Journal of Maize Sciences,2006,14(2):006-009. |
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| Authors: | ZHOU Zhu-hu ZHANG Feng-yun XU Li-hu QIU Deng-lin XING Yan-ju YIN Wei-jun |
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| Institution: | The Institute for Application of Atomic Energy, Shandong Academy Qf A gricultural Sciences, Jinan 250100, China |
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| Abstract: | The DNA from Zea Diploperennis was introduced into the maize self-bred line(Huangzao 4) through pollen tube pathway technique.The variation in biological characters of offspring was investigated in continuing three years.The results indicated that the transgenic offspring showed a lower seedling emergence percentage,delayed germinating time,a decreased plant height and ear height.The spike(spicula) characteristics of transformants were also changed.A great range of variations appeared in their offspring.The transformation of exogenous DNA into Huangzao 4 enlarged the variations range of agronomic characters of offspring and generated diverse germplasm materials which were benefit to breeding and provided us much more chances to select excellent varieties. |
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| Keywords: | Maize self-bred line Zea Disploperennis DNA transformation Agronomic characters Biotechnology Pollen tube pathway |
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