国家种质广州甘薯圃病毒种类鉴定及分析

为明确引起国家种质广州甘薯资源圃中病毒病的病毒种类及优势种,为甘薯种质安全保存提供支持,2017年从甘薯资源圃中未脱毒更新的盆栽苗和大田苗中采集155份具有不同病毒病症状的甘薯资源样品,利用PCR和RT-PCR检测技术对这些样品进行了17种病毒的分子检测.155份样品均有病毒检出,包括甘薯羽状斑驳病毒Sweet pot...

Identification and analysis of viruses in the national germplasm nursery for sweet potato(Guangzhou)

In order to investigate the virus and dominant virus species causing sweet potato virus disease in the national sweet potato germplasm Guangzhou resource nursery, provide support for the safe preservation of resources, a total of 155 samples with viral symptoms were collected from potted seedlings and field seedlings in nursery in 2017. Molecular detection of viruses in the samples were carried out by PCR or RT PCR using primers for seventeen viruses. The results showed that nine virus species were detected, including Sweet potato feathery mottle virus (SPFMV), Sweet potato chlorotic stunt virus (SPCSV), Cucumber mosaic virus (CMV), Sweet potato chlorotic fleck virus (SPCFV), Sweet potato caulimo like virus (SPCV), Sweet potato latent virus (SPLV), Sweet potato vein mosaic virus (SPVMV), Sweet potato virus 2 (SPV2) and Sweet potato leaf curl virus (SPLCV). Among which, SPCFV had the highest detection rate of 98.1%, and SPCV had the lowest detection rate of 0.6%. The detection rate of RNA viruses was 99.4%, and that of DNA viruses was 94.2%. One hundred and fifty four samples (99.4%) were found to be infected by two or above viruses. Specifically, co infection by 4-6 kinds of viruses were major type, accounting for 70.7%. A total of eight viruses were detected in four samples (2.6%). Combined infection contained SPFMV and SPCSV was the most common, accounting for 54.2%. In short, some germplasm resources of sweet potato have been infected by different viruses with high infection rates and mixed infection was dominant. The tolerance of different resources to the virus is obviously different under the same growth environment.