广西南宁甘蔗赤条病调查及分子检测

2020年在广西南宁市金光农场发现'桂糖58'和'柳城05-136'疑似感染甘蔗赤条病,为明确其病原,本研究对不同甘蔗品种进行了发病率调查,并采集病样进行了PCR检测分析.田间调查结果表明:不同品种自然发病率不同,'桂糖58'发病率为29％～52.33％,发病严重田块平均发病率为49.67％,发病中等田块平均发病率为3...

Investigation and molecular detection of sugarcane red stripe caused by Acidovorax avenae subsp. avenae in Nanning, Guangxi

In 2020,suspected sugarcane red stripe disease was found in‘Guitang 58’and‘Liucheng 05-136’in Jinguang farm,Nanning city,Guangxi.In order to identify the pathogen,we investigated the incidence of different sugarcane varieties,and collected disease samples for PCR molecular detection and sequencing analysis.Field survey results showed that the incidence of different varieties were different.The disease incidence in‘Guitang 58’was 29%-52.33%,the average disease incidence of severely,moderately and lighter affected fields was 49.67%,33.67%,29.89%,respectively.The disease incidence in‘Liucheng 05-136’was 2%-27.67%,the average disease incidence of severely,moderately and lighter affected fields was 21.67%,21.22%and 4.67%,respectively.PCR analysis showed that 19 samples amplified a 550 bp specific band with a positive detection rate of 95%.And the obtained sequences had 100%homology and were on the same branch with Acidovorax avenae subsp.avenae in phylogenetic tree,which confirmed that the tested samples were sugarcane red stripe disease caused by A.avenae subsp.avenae.The above results showed that‘Guitang 58’was highly susceptible variety and‘Liucheng 05-136’was susceptible variety.In the future,corresponding prevention and control measures should be actively taken to prevent the spread of the bacterial disease and ensure the safe and sustainable development of sugar industry in China.