滇重楼根水浸液对四种园林植物的化感作用研究

为了在保健型园林中可以科学合理地应用滇重楼,采用室内生物测定法研究了滇重楼(Paris polyphylla var. Yunnanensis)根水浸液(0、0.02、0.04、0.06、0.08 g·mL^-1)对4种园林植物种子萌发及幼苗生长的化感作用。结果表明,滇重楼根水浸液对4种园林植物种子萌发及幼苗生长有显著影响,且4种园林植物对根水浸液的响应不同。滇重楼根水浸液对4种园林植物种子萌发和幼苗生长主要表现为化感抑制作用,且随着浸提液浓度的升高而逐渐增强,仅孔雀草(Tagetes patula)在浸提液浓度≤0.04 g·mL^-1时表现一定的促进作用;4种园林植物对滇重楼根水浸液化感作用综合效应的敏感性大小依次为白车轴草(Trifolium repen)>鼠尾草(Salvia japonica)>高羊茅(Festuca arundinacea)>孔雀草;滇重楼根水浸液对4种园林植物幼苗叶绿素含量基本表现为抑制作用,仅孔雀草在0.02 g·mL^-1处理下表现为促进作用;SOD、CAT活性随滇重楼根水浸液浓度升高而先增后降,POD活性和MDA含量随浓度升高而增加,仅孔雀草在浸提液浓度≤0.04 g·mL^-1时MDA含量减少。因此,滇重楼根水浸液先使4种园林植物遭受氧化胁迫,进而表现出化感抑制作用。

Allelopathy of aqueous extracts from Paris polyphylla var. Yunnanensis roots on four ornamental plants

Different concentrations of aqueous extracts from Paris polyphylla var. Yunnanensis roots were used for allelopathic study on seed germination and seedling growth of Tagetes patula, Salvia japonica, Trifolium repens, Festuca arundinacea by the laboratory bioassay in order to provide certain scientific basis for reasonable utilization of Paris polyphylla var. Yunnanensis in garden. The results showed that aqueous extracts from Paris polyphylla var. Yunnanensis roots had significant effects on seed germination and seedling growth of four ornamental plants. But the effects of different concentrations of aqueous extracts on four garden plants were quite different. The aqueous extracts from Paris polyphylla var. Yunnanensis roots had inhibitory effects on seed germination and seedling growth of four ornamental plants. And when the concentration of aqueous extracts increased, the inhibition enhanced gradually. On the contrary, lower treatments (≤0.04 g·mL^-1) increased seed germination and seedling growth of Salvia japonica in a degree. The comprehensive sensitivity of the allelopathic effect on the four ornamental plants of aqueous extracts from Paris polyphylla var. Yunnanensis roots was in the order of Tagetes patula>Trifolium repens>Festuca arundinacea>Salvia japonica. The aqueous extracts from Paris polyphylla var. Yunnanensis roots had inhibitory effects on chlorophyll content of four ornamental plants. On the contrary, aqueous extracts at a concentration of 0.02 g·mL^-1 were found to be facilitated to chlorophyll content for Salvia japonica. When the concentration of aqueous extract increased, the activities of superoxide dismutase (SOD) and catalase (CAT) increased at first and decreased later, and peroxidase (POD) activity and malondialdehyde (MDA) content increased. On the contrary, lower treatments (≤0.04 g·mL^-1) increased malondialdehyde content of Salvia japonica in a degree. Therefore, aqueous extracts from Paris polyphylla var. Yunnanensis roots make four plants suffer from oxidative stresses firstly. Then, they show an allelopathic inhibition.