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| 抗犬瘟热病毒荧光标记单抗的制备和初步鉴定(英文) | |
| 引用本文： | 苏建青,褚秀玲,杨松涛,夏咸柱,岳妙姝. 抗犬瘟热病毒荧光标记单抗的制备和初步鉴定(英文)[J]. （《农业科学与技术》）编辑部, 2009, 0(1) |
| 作者姓名： | 苏建青 褚秀玲 杨松涛 夏咸柱 岳妙姝 |
| 作者单位： | 聊城大学农学院;中国人民解放军军事医学科学院军事兽医研究所;长春理工大学生命科学技术学院; |
| 基金项目： | Supported by Science and Technology Foundation of PLA General Lo-gistics Department(06G138)~~ |
| 摘 要： | [Objective] The aim of the present study was to develop a direct immunofluorescence method for the diagnosis of canine distemper (CD) with FITC-conjugated monoclonal antibodies (FITC-McAb). [Method] The McAb against CDV, designated as CE3, was purified with protein G and labeled with FITC through agitation method. After purification and identification, the optimal working concentration of FITC-labeled CE3 was determined. Then 61 clinical samples of suspected canine distemper were detected by direct immunofluorescence assay. [Result] The absorption test, blocking test and specificity test showed that the labeled antibody had high specificity and sensitivity, but didn't have cross reaction with canine parvovirus (CPV), canine parainfluenza virus (CPIV), canine adenovirus (CAV) and rabies virus (RV). The optimal working concentration was 1∶80. The positive rate of clinical suspected samples was 48%. [Conclusion] The direct immunofluorescence assay developed in this study was rapid, specific and convenient, and had great significance for the early diagnosis of canine distemper. |
| 关 键 词： | 制备 单克隆抗体 阴性对照 荧光素 特异性 免疫荧光试验 感染细胞 犬瘟热病毒 单抗 荧光抗体 |
Preparation and Preliminary Identification of Fluorescein Labeled Monoclonal Antibody against Canine Distemper Virus |
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| SU Jian-qing,CHU Xiu-ling,YANG Song-tao,XIA Xian-zhu,YUE Miao-shu. College of Agriculture,Liaocheng University,Liaocheng . Preparation and Preliminary Identification of Fluorescein Labeled Monoclonal Antibody against Canine Distemper Virus[J]. 农业科学与技术(英文版)Agricultural Science ＆ Technology, 2009, 0(1) | |
| Authors: | SU Jian-qing CHU Xiu-ling YANG Song-tao XIA Xian-zhu YUE Miao-shu. College of Agriculture Liaocheng University Liaocheng |
| Affiliation: | SU Jian-qing1,CHU Xiu-ling1,YANG Song-tao2,XIA Xian-zhu2,YUE Miao-shu31. College of Agriculture,Liaocheng University,Liaocheng 252059,2. Institute of Military Veterinary,Academy of Military Medical Sciences of PLA,Changchun 130062,3. College of Life Science , Technology,Changchun University of Science , Technology |
| Abstract: | [Objective] The aim of the present study was to develop a direct immunofluorescence method for the diagnosis of canine distemper (CD) with FITC-conjugated monoclonal antibodies (FITC-McAb). [Method] The McAb against CDV, designated as CE3, was purified with protein G and labeled with FITC through agitation method. After purification and identification, the optimal working concentration of FITC-labeled CE3 was determined. Then 61 clinical samples of suspected canine distemper were detected by direct immunofl... |
| Keywords: | Canine distemper virus Direct immunofluorescence assay Monoclonal antibody |
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