糯玉米单倍体诱导及其加倍研究

以吉诱101为父本诱导11份糯玉米材料,研究花丝长度、授粉时期对糯玉米单倍体诱导率的影响,比较不同处理方法对糯玉米单倍体的加倍效果。结果表明,糯玉米单倍体平均诱导率,长花丝时期授粉为13.47%,短花丝时期授粉为5.75%;伏后期授粉为12.86%,伏期授粉为6.22%。加倍试验结果表明,平均散粉率,浸芽法(10.66%)浸根法(6.20%)浸种法(4.34%)自然加倍法(1.05%),其中,浸芽法加倍效果最好。

Study on Haploid Inducing and Doubling of Waxy Maize

Eleven waxy maize materials were induced by haploid inducers (Jiyou 101) to study the effect of silk length, pollinating date on haploid inducing rate and different approaches on haploid doubling. The results of haploid inducing indicated that, the average haploid inducing rate of long silk pollination was 13.47%, short silk pollination was 5.75%, after summer pollination was 12.86%, summer pollination was 6.22%. The results of haploid doubling indicated that, the average loose powder rate of dipping shoot was 10.66%, dipping root was 6.20%, dipping seed was 4.34%, spontaneous was 1.05%, the best way of haploid doubling was dipping shoot.