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| 锦鲤疱疹病毒囊膜蛋白ORF132的克隆及分析 | |
| 引用本文： | 刘振兴,柯浩,孟轩,林敏,陈棠堂,颜远义.锦鲤疱疹病毒囊膜蛋白ORF132的克隆及分析[J].广东农业科学,2011,38(6):134-136. |
| 作者姓名： | 刘振兴 柯浩 孟轩 林敏 陈棠堂 颜远义 |
| 作者单位： | 1. 广东省农科院兽医研究所,广东广州510640;广东省兽医公共卫生公共实验室,广东广州510640 2. 中山市海洋与渔业局,广东中山,528400 3. 广东省水生动物疫病预防控制中心,广东广州,510222 |
| 基金项目： | 广东省农业科学院兽医研究所所长基金(2009SJJ05); 广东省2009年省级鱼病防治专项基金(2130108) |
| 摘 要： | 提取感染锦鲤疱疹病毒(KHV)的锦鲤(Cyprinus carpio koi)肾脏组织DNA,通过PCR扩增了KHV ORF132基因。该基因全长513 bp,所编码的蛋白包含170个氨基酸,分子量18.5 ku,等电点8.11,有信号肽以及2个潜在的O糖基化位点。应用DNA Star程序,通过综合分析二级结构柔性区、蛋白的亲水性、表面可能性、抗原性指数,预测KHV ORF132蛋白主要B细胞表位区段位于Leu40～Ala45、Asn53～Pro66、Arg97～Thr118、Ala125～Pro136、Glu140～Arg145、Asn160～Arg170。 |
| 关 键 词： | 锦鲤疱疹病毒 囊膜蛋白 B细胞表位 |
Characterization and expression of a truncated Koi Herpesvirus envelope protein ORF132 |
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| LIU Zhen-xing,KE Hao,MENG Xuan,LIN Min,CHEN Tang-tang,YAN Yuan-yi.Characterization and expression of a truncated Koi Herpesvirus envelope protein ORF132[J].Guangdong Agricultural Sciences,2011,38(6):134-136. | |
| Authors: | LIU Zhen-xing KE Hao MENG Xuan LIN Min CHEN Tang-tang YAN Yuan-yi |
| Institution: | LIU Zhen-xing1,2,KE Hao1,MENG Xuan1,LIN Min1,CHEN Tang-tang3,YAN Yuan-yi4 (1.Institute of Veterinary Medicine,Guangdong Academy of Agricultural Sciences Guangzhou 510640,China,2.Guangdong Common Lab of Veterinary Public Health,Guangzhou 510640,3.Zhongshan Oceanic and Fishery Bureau,Zhongshan 528400,China 4.Guangdong Provinical Aquatic Animal Disease Control Center,Guangzhou 510222,China) |
| Abstract: | Koi Herpesvirus ORF132 gene was amplified by PCR using template DNA extracted from the kidney of Cyprinus carpio koi infected Koi Herpesvirus(KHV).The full length of KHV ORF132 gene was 513 bp encoding an envelope protein composing 170 amino acids.The predicted molecular weight of KHV ORF132 protein was 18.5 ku,and its estimated isoelectric point was 8.11.A signal peptide and 2 potential O-glycosylation sites were found in this protein.Based on the analysis of the flexibility in second structure,hydrophilic... |
| Keywords: | KHV envelope protein B cell epitope |
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