陆地棉细胞色素P450超家族基因GhCYP85A2-1的克隆与功能分析

【目的】研究细胞色素P450超家族CYP85A亚家族基因在棉花株高发育中的生物学功能,为陆地棉株高分子育种提供理论依据和基因资源。【方法】 采用同源克隆方法,从陆地棉中克隆CYP85A家族基因GhCYP85A2-1,利用烟草脆裂病毒(Tobacco rattle virus, TRV)诱导的基因沉默技术(Virus-induced gene silencing, VIGS)构建GhCYP85A2-1基因沉默载体,利用qRT-PCR技术检测侵染棉花幼苗的基因表达量。【结果】 GhCYP85A2-1基因沉默植株的株高显著降低,基因的表达量也显著下降。【结论】 GhCYP85A2-1基因在棉花株高发育过程中起到重要调控作用。

Cloning and Functional Analysis of Cytochrome P450 Superfamily Gene GhCYP85A2-1 in Upland Cotton

【Objective】 The principal objective is to investigate the biological function of CYP85A subfamily genes of the cytochrome P450 superfamily in the development of cotton plant height in the hope of providing a scientific basis for molecular breeding of upland cotton plants.【Method】 A CYP85A family gene GhCYP85A2-1 was cloned from upland cotton by homologous cloning. Tobacco rattle virus (TRV) induced gene silencing technology (Virus-induced gene silencing, VIGS) was used to construct GhCYP85A2-1 gene silencing vector, total RNA was extracted, and gene expression of GhCYP85A2-1 in infecting cotton seedlings was investigated by qRT-PCR.【Result】 The study found that the height of GhCYP85A2-1 gene silenced plants was significantly reduced, and so was the gene expression.【Conclusion】 GhCYP85A2-1 gene plays an important regulatory role in the development of cotton plant height.