油茶品种ISSR指纹图谱数据库的建立

为探究油茶品种的遗传多样性并了解其遗传关系，选用11条引物，对来自秦巴山区的32个油茶品种进行分子指纹分析，研究不同品种间的遗传距离和遗传相似性。筛选出多态性较高的11条ISSR引物进行PCR扩增，共扩增出86个条带，其多态性条带为51条，多态性位点比率为60.28%。利用Visual Basic 和Access 软件进行数据库编程，创建一个数据库，对11对引物对样品的扩增结果赋值进行排列，通过赋值对不同油茶种质进行鉴别。

Construction of Molecular Fingerprinting Database for Camellia oleifera by ISSR Markers

In order to assess the genetic diversity of Camellia oleifera and to understand their genetic relationship, molecular fingerprinting analysis was used to determine the genetic similarity and genetic distance of 32 Camellia oleifera varieties from Qinba mountain with 11 ISSR primers. The results showed that a total of 86 allelic loci were detected, with 51 polymorphic loci and polymorphic loci ratio was 60.28 %. Based on the electrophoresis results, a database was established with the soft wares of Visual Basic and Access, and the amplified bands by the selected 11 primers were digitized and input into the database, which became the gene identification number of Camellia oleifera. Thus, the Camellia oleifera varieties could be identified by their molecular identification.