Rat mesenchymal stem cells differentiate into neuron-like cells induced by berberine in vitro |
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Authors: | XIANG Ping LI Hai-biao |
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Institution: | Department of Histology and Embryology, Medical School of Sun Yat-sen University,Guangzhou 510089,China |
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Abstract: | AIM: To investigate whether berberine can induce rat mesenchymal stem cells (MSCs) to differentiate into neuron -like cells in vitro. METHODS: MSCs were separated from young rat femurs marrow and expanded in culture medium. MSCs were induced to differentiate by berberine. The morphological changes of MSCs were evaluated by light microscope.Neuron-spcific enolase (NSE), neurofilament (NF), glial fibrillary acidic protein (GFAP) were detected by immunohistochemistry. RESULTS: Induced by berberine for 8 hours,MSCs exhibited neurotype . The expression of NSE and NF in the neuron-like cells was positive, but the glial astrocyte marker GFAP didn't express. CONCLUSION: Berberine may induce adult rat MSCs to differentiate into neuron-like cells in vitro. |
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Keywords: | Mesenchymal stem cells Neuron Berberine Rats |
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