| 重组人酸性成纤维细胞生长因子对神经细胞损伤的保护作用 |
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| 引用本文： | 曹定国,周汝滨,廖霞,陈小平,李校堃. 重组人酸性成纤维细胞生长因子对神经细胞损伤的保护作用[J]. 湛江医学院学报, 2007, 25(2): 126-128 |
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| 作者姓名： | 曹定国 周汝滨 廖霞 陈小平 李校堃 |
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| 作者单位： | 广东医学院遗传学研究室,广东湛江,524023;暨南大学药学院,广东广州,510632 |
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| 基金项目： | 广东医学院校科研和教改项目 |
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| 摘 要： | 目的建立谷氨酸损伤模型并探讨重组人酸性成纤维细胞生长因子(rhaFGF)对谷氨酸损伤神经细胞的干预作用。方法利用流式细胞仪观察rhaFGF对正常培养的神经细胞细胞周期的影响,建立细胞损伤模型,将实验分空白组、模型组和rhaFGF保护组,通过形态观察以及MTT法检测各实验组的细胞活力。结果rhaFGF能促进DNA的合成,谷氨酸对细胞损伤程度随浓度的增大而增大,rhaFGF能够拮抗谷氨酸的神经毒。结论rhaFGF能促进神经细胞生长同时拮抗谷氨酸兴奋性毒性而发挥神经保护作用。
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| 关 键 词： | 酸性成纤维细胞生长因子 SH-SY5Y细胞 谷氨酸 细胞活力 |
| 文章编号： | 1005-4057(2007)02-0126-03 |
| 修稿时间： | 2006-08-30 |
Protective effects of rhaFGF on cultured neurons injured by glutamate |
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| CAO Ding-guo,ZHOU Ru-bin,LIAO Xia,CHEN Xiao-ping,LI Xiao-kun. Protective effects of rhaFGF on cultured neurons injured by glutamate[J]. Journal of Guangdong Medical College, 2007, 25(2): 126-128 |
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| Authors: | CAO Ding-guo ZHOU Ru-bin LIAO Xia CHEN Xiao-ping LI Xiao-kun |
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| Affiliation: | 1. Department of Medical Genetim, Guangdong Medical College , Zhanjiang 524023 , China; 2. Phamarceutical College , Jinan Univercity , Guangzhou 510632 , China |
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| Abstract: | Objective To study the protective effects of rhaFGF on cultured neuron SH-SY5Y iniured by glutamate. Methods The neuron SH-SY5Y damaged model in vitro was established with the treatment of ecltatory amino acid; cell cycle was analyzed with flowcytometer; morphological change was observed with microscope. Cell survival status was measured with MTT method. Results rhaFGF promoted the DNA synthesis significantly; cellular damage increased with the treatment of increasing gluamate dosage, rhaFGF improved the survival rate of SH-SY5Y and inhibited the cell damage induced by excitatory amino acid. Conclusion rhaFGF could improve the survival rate of neurons. |
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| Keywords: | aFGF SH-SYSY glutamate cell vitality |
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