| 甜瓜蔗糖合成酶基因(CmSS1)反义表达载体的构建及遗传转化 |
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| 引用本文： | 闻小霞,赵荷仙,刘刚,周爱凤,张桂梅. 甜瓜蔗糖合成酶基因(CmSS1)反义表达载体的构建及遗传转化[J]. 山东农业科学, 2013, 45(4) |
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| 作者姓名： | 闻小霞 赵荷仙 刘刚 周爱凤 张桂梅 |
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| 作者单位： | 1. 聊城市农业科学研究院,山东聊城,252000
2. 聊城市东昌府区梁水镇农业技术推广站,山东聊城,252000 |
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| 摘 要： | 以BamHⅠ和SalⅠ双酶切pMD18-T-CmSS1和表达载体pBI121,再用T4 DNA连接酶将回收的目的片段反向与pBI121连接。结果证明,CmSS1反向插入到pBI121中,得到了pBI121-CmSS1的重组质粒;利用农杆菌介导法将反义CmSS1基因转化甜瓜,经PCR检测,得到5株转基因植株。这为以后研究甜瓜蔗糖合成酶的活性调节机制及通过基因工程手段研究甜瓜SS的活性奠定了基础。
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| 关 键 词： | 甜瓜 蔗糖合成酶 反义表达载体 遗传转化 |
Construction of CmSS1 Antisense Expression Vector and Its Transformation into Muskmelon |
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| Wen XiaoXia , Zhao HeXian , Liu Gang , Zhou AiFeng , Zhang GuiMei. Construction of CmSS1 Antisense Expression Vector and Its Transformation into Muskmelon[J]. Shandong Agricultural Sciences, 2013, 45(4) |
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| Authors: | Wen XiaoXia Zhao HeXian Liu Gang Zhou AiFeng Zhang GuiMei |
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| Abstract: | The pMD18-T-CmSS1 and the expression vector pBI121 were digested respectively with BamHⅠand SalⅠ,and then the target fragments were connected by T4 DNA ligase.The results showed that the CmSS1 gene was successfully inserted into the plant expression vector pBI121 and the antisense expression vector pBI121-CmSS1 was obtained.The anti-CmSS1 was transformed to muskmelon by Agrobacterium-mediated,and five transgenic plants were obtained by PCR detection.This research laid foundations for further research of regulation mechanisms of muskmelon SS activity by genetic engineering. |
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| Keywords: | Muskmelon Sucrose synthase Antisense expression vector Genetic transformation |
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