| 槭树叶片类黄酮糖基转移酶基因RT-PCR扩增体系优化 |
| |
| 引用本文： | 尹燕雷,苑兆和,招雪晴,冯立娟,杨尚兵,李云.槭树叶片类黄酮糖基转移酶基因RT-PCR扩增体系优化[J].山东农业科学,2013,45(3). |
| |
| 作者姓名： | 尹燕雷 苑兆和 招雪晴 冯立娟 杨尚兵 李云 |
| |
| 作者单位： | 山东省果树研究所,山东泰安,271000 |
| |
| 基金项目： | 山东省自然科学基金项目 |
| |
| 摘 要： | 以槭树"秋天火焰"变色期秋季叶片为试材,采用改良CTAB法提取叶片总RNA,利用GenBank中相关物种的UFGT基因序列设计特异引物,进行RT-PCR扩增并对RT-PCR反应体系中的引物、退火温度和循环次数进行优化,建立了槭树叶片UFGT基因的RT-PCR扩增反应体系。
|
| 关 键 词： | 槭树 UFGT RT-PCR |
Optimization of RT-PCR Amplification System for UFGT Gene in Maple Leaves |
| |
| Yin YanLei
,
Yuan ZhaoHe
,
Zhao XueQing
,
Feng LiJuan
,
Yang ShanBing
,
Li Yun.Optimization of RT-PCR Amplification System for UFGT Gene in Maple Leaves[J].Shandong Agricultural Sciences,2013,45(3). |
| |
| Authors: | Yin YanLei Yuan ZhaoHe Zhao XueQing Feng LiJuan Yang ShanBing Li Yun |
| |
| Abstract: | The maple cultivar 'Autumn Blaze' was used as the experiment material in the article.Total RNA was extracted from the autumn leaves of maple tree by improved CTAB method.Specific primers were designed according to the conservative regions of UFGT gene from some related species listed in the GenBank database.The RT-PCR amplification was carried out.The specific primers,annealing temperature and cycle number were optimized.An optimized,effective and specific RT-PCR system for amplification of UFGT gene was established. |
| |
| Keywords: | Maple UFGT RT-PCR |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
