| 杨树葡萄糖-6-磷酸脱氢酶（G6PDH）基因启动子的克隆与分析 |
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| 引用本文： | 林元震,张志毅,郭海,刘纯鑫,陈晓阳.杨树葡萄糖-6-磷酸脱氢酶（G6PDH）基因启动子的克隆与分析[J].广西农业生物科学,2009(3):445-449. |
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| 作者姓名： | 林元震 张志毅 郭海 刘纯鑫 陈晓阳 |
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| 作者单位： | 华南农业大学林学院;北京林业大学生物学院;水利部水土保持植物中心; |
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| 基金项目： | 国家自然科学基金项目(30771759);;国家十一五科技支撑计划专题(2006BAD07A04)共同资助 |
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| 摘 要： | 葡萄糖-6-磷酸脱氢酶是磷酸戊糖途径的关键性调控限速酶，其主要功能是为脂肪酸合成、氮还原和谷胱甘肽等生物分子合成提供还原力NADPH，也为核酸合成提供戊糖；此外，还参加非生物逆境胁迫应答反应。因此，G6PDH对植物的生长发育起着非常重要的作用。本文利用甜杨G6PDH基因和毛果杨基因组序列，通过PCR获得了甜杨G6PDH基因上游1400bp的序列。序列分析结果表明，该序列具有启动子的基本元件TATA-box、CAAT-box。此外，还包含多个胁迫诱导元件，如低温诱导元件LTR，盐诱导元件GT-1，抗冻、缺水、脱落酸、抗寒元件MYB和MYC，以及光响应元件L-box、G-box、3AF-1、TC丰富区等。
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| 关 键 词： | 杨树 葡萄糖-6-磷酸脱氢酶 启动子 |
Isolat ion and Analysis of Glucose-6-phosphate Dehydrogenase(G6PDH) Promoter from Poplar |
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| Lin Yuanzhen , Zhang Zhiyi Guo Hai Liu Chunxin Chen Xiaoyang College of Forestry,South China Agricultural University,Guangzhou, Biological Institute,Beijing Forestry University,Beijing , Plant Materials for Soil , Water Conservation,Ministry of Water Resources,Beijing.Isolat ion and Analysis of Glucose-6-phosphate Dehydrogenase(G6PDH) Promoter from Poplar[J].Journal of Guangxi Agricultural and Biological Science,2009(3):445-449. |
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| Authors: | Lin Yuanzhen Zhang Zhiyi Guo Hai Liu Chunxin Chen Xiaoyang College of Forestry South China Agricultural University Guangzhou Biological Institute Beijing Forestry University Beijing Plant Materials for Soil Water Conservation Ministry of Water Resources Beijing |
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| Institution: | Lin Yuanzhen 1,2 Zhang Zhiyi 2 Guo Hai 3 Liu Chunxin 1 Chen Xiaoyang 1 1 College of Forestry,South China Agricultural University,Guangzhou,510642,2 Biological Institute,Beijing Forestry University,Beijing 100083,3 Plant Materials for Soil , Water Conservation,Ministry of Water Resources,Beijing,100038 |
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| Abstract: | Glucose-6-phosphate dehydrogenase(G6PDH) is the first and main regulated enzyme of the oxidative pentose phosphate pathway(OPPP), and it is a major source of reductant(NADPH) for biosynthetic processes and carbon skeletons for nucleotides synthesis.Moreover, its exepression is also involved in different abotic stresses.Therefore G6PDH plays important roles in the growth and development of plants.A 5'-flanking 1 400 bp proximal region of G6PDH gene was isolated from the genomic DNA of Populus suaveolens by P... |
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| Keywords: | Poplar （Populus suaveolens） Glucose-6-phosphate dehydrogenase （G6PDH） Promoter |
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