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| 两个牡丹杂交系种子胚培养技术研究 | |
| 引用本文： | 曾端香,袁涛,王莲英,王福.两个牡丹杂交系种子胚培养技术研究[J].热带农业科学,2011,31(3):8-12. |
| 作者姓名： | 曾端香 袁涛 王莲英 王福 |
| 作者单位： | 1. 国家林业局管理干部学院,北京,102600;北京林业大学园林学院,北京,100083 2. 北京林业大学园林学院,北京,100083 3. 牡丹芍药科技开发有限公司,河南洛阳,471500 |
| 基金项目： | 国家林业局重点课题"牡丹新优品种繁育及区域化实验"项目资助 |
| 摘 要： | 为保存和扩繁牡丹杂交系种质资源,提供牡丹组培苗的菌根化供试体系,本试验筛选牡丹杂交系‘Z1-10-3’和‘Z1-WM’的种子,取种胚进行胚培养,建立了牡丹胚培养无菌体系。结果表明：成熟种子经70%酒精2min与0.1%HgCl20 min表面消毒后去外种皮切开胚乳直接取胚接种,3 d时启动率达76.7%,比直接用种子和去外种皮的种子接种启动时间提前30～50 d。胚根苗直接培养的最佳培养基和培养条件为：1/2MS＋IBA1.0mg/L＋AC0.4g/L＋Sugar30g/L,在自然弱光照下培养。 |
| 关 键 词： | 牡丹 杂交系种子 胚培养 最佳培养基 |
Embryo-culture of 2 Hybrid Strains Seeds of Tree Peony |
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| ZENG Duanxiang,YUAN Tao,WANG Lianying,WANG Fu.Embryo-culture of 2 Hybrid Strains Seeds of Tree Peony[J].Chinese Journal of Tropical Agriculture,2011,31(3):8-12. | |
| Authors: | ZENG Duanxiang YUAN Tao WANG Lianying WANG Fu |
| Institution: | ZENG Duanxiang1,2) YUAN Tao2) WANG Lianying2) WANG Fu3)(1 State Academy of Forestry Administration,Beijing 102600,China,2 College of Landscape Architecture,Beijing Forestry University,Beijing 100083,3 Peony Technology Development Co.Ltd.,Luoyang,Henan 471500,China) |
| Abstract: | In order to conserve and multiply germplasm resources of hybrid strain of tree peony,and provide tissue culture seedling with arbuscular mycorrhizae,the germ-free system of embryo-culture was constructed in hybrid seeds of tree peony('Z1-10-3','Z1-WM').Firstly,the seed surface of tree peony were sterilized by 70% alcohol 2 min and 0.1% HgCl2 20 min.Secondly,the seed coat and endosperm were wiped off.Then the embryo was used to cultivate.About 76.7% of embryos were germinated after 3 d.The starting time of g... |
| Keywords: | tree peony hybrid seeds embryo-culture optimal media |
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