拔毛·剃毛对绵羊皮肤中GHR·IGF-1·IGF-1R·KAP3.2和KAP6-1基因表达的影响

选取36只新疆细毛羊随机分成6组进行拔毛、剃毛处理,于第0、3、6、9、12、50天采集皮肤样品,用反转录多聚酶链式反应(RT-PCR)方法,定量分析绵羊皮肤中生长激素受体(GHR)、胰岛素样生长因子1(IGF-1)和胰岛素样生长因子1型受体(IGF-1R)、角蛋白关联蛋白KAP3.2和KAP6-1mRNA的相对丰度。实验结果显示,与对照组相比,剃毛可显著提高IGF-1的表达量,但对GHR、IGF-1R、KAP3.2和KAP6-1的表达量均无明显的影响;而拔毛对皮肤中GHRI、GF-1I、GF-1R、KAP3.2和KAP6-1基因表达均无明显影响。

Effect of Plucking or Shaving on Expression of GH Receptor, IGF-1 ,IGF-1 Receptor, KAP3.2 and KAP6-1 mRNA in the Skin of Sheep

Thirty six healthy and similar Xinjiang sheep plucked and shaved respectively were selected and divided into six groups at random.Treatment skin tissue of sheep were sampled respectively for 0,3,6,9,12 and 50 days and the skin expression of growth hormone receptor(GHR),insulin-like growth factor1(IGF-1),insulin-like growth factor receptor(IGF-R),KAP3.2 and KAP6-1 mRNA were measured by RT-PCR.The results indicated that the relative abundance of IGF-1 mRNA in shaved skin was improved evidently,compared with control,but there was no significant differences in expression of GHR,IGF-1R,KAP3.2 and KAP6-1;while the relative abundance of GHR,IGF-1,IGF-1R,KAP3.2 and KAP6-1 mRNA in plucked skin was no evident change respectively?