| 两种外源激素在白掌组织培养不同阶段的剂量研究 |
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| 引用本文： | 崔锡明,林思诚,柯沛强.两种外源激素在白掌组织培养不同阶段的剂量研究[J].广东林业科技,2004,20(2):20-22. |
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| 作者姓名： | 崔锡明 林思诚 柯沛强 |
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| 作者单位： | 1. 茂名市科学技术局
2. 广东省茂名市林业科学研究所,广东,茂名,525000 |
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| 摘 要： | 文章研究了不同浓度的细胞分裂素6-BA对白掌外植体诱导、愈伤组织增殖、不定芽增殖及生长素IBA对诱导生根的影响。结果表明：初代培养基以1／2MS CM100ml／L 蔗糖30g／L 琼脂6g／L 6-BA 1.0mg／L效果最好；在培养基MS NAA 0．1mg／L 蔗糖30g／L 琼脂6g／L中添加6-BA 1.0mg／L愈伤组织和不定芽的增殖倍数最高；在培养基1／2MS 活性炭2g／L 蔗糖30g／L 琼脂6g／L中添加2．0mg／L IBA可显著提高生根率。
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| 关 键 词： | 白掌 组织培养 培养基 激素 6-BA IBA 剂量 观赏植物 |
The Concentration of 6-BA and IBA in Distinct Stages of Spathiphyllum kochii Tissue Culture |
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| Cui Ximing,Lin Sicheng,Ke Peiqiang.The Concentration of 6-BA and IBA in Distinct Stages of Spathiphyllum kochii Tissue Culture[J].Forestry Science and Technology of Guangdong Province,2004,20(2):20-22. |
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| Authors: | Cui Ximing Lin Sicheng Ke Peiqiang |
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| Abstract: | This article study the effect of different 6 BA concentration to the induction rate of explants, the proliferation of callus, the proliferation of adventitious bud in Spathiphyllum kochii tissue culture. The effect of different IBA concentration to the rooting are also studied. The result showed that induction rate of explants was highest when added 1.0 mg/L 6 BA to medium 1/2MS+CM100 ml/L+sugar 30 g/L+agar agar 6 g/L, added 6 BA 1.0 mg/L to medium MS+NAA 0.1 mg/L+sugar 30 g/L+agar agar 6 g/L was good for raising rate of increase of callus and adventitious root, added 2.0 mg/L IBA to medium 1/2MS+ activated carbon 2.0 mg+sugar 30 g/L+agar agar 6 g/L could raising rooting rate strikingly. |
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| Keywords: | Spathiphyllum kochii tissue culture culture medium hormone 6-BA IBA |
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