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| 蟆叶秋海棠离体培养的研究 | |
| 引用本文： | 何勇,何爱平,禹丹青,田志宏. 蟆叶秋海棠离体培养的研究[J]. 湖北农业科学, 2009, 48(8) |
| 作者姓名： | 何勇 何爱平 禹丹青 田志宏 |
| 作者单位： | 长江大学生命科学学院,湖北,荆州,434025;武汉大学生命科学学院,武汉,430072 |
| 基金项目： | 湖北省重点科技发展汁划项月，长江大学科研基金 |
| 摘 要： | 以蟆叶秋海棠(Begonia rex)的幼嫩叶片为外植体,采用L25(55)正交设计,研究了不同生长调节剂和培养基对愈伤组织诱导的影响.结果表明,培养基的类型对愈伤组织诱导的影响极大,生长调节荆对愈伤组织诱导有一定的影响,愈伤组织在不添加任何生长调节剂的培养基上即可进行分化.蟆叶秋海棠最佳愈伤组织诱导培养基组合为1/2 MS+0.5～1.5 mg·L-1 KT+0.5 mg·L-16-BA+0.5～2.0 mg·L-1α-NAA+0.8%琼脂+3%蔗糖,愈伤组织诱导率可高达100%.生根培养基为1/2 MS+0.2 mg·L-1α-NAA+0.8%琼脂+3%蔗糖,生根率高达100%. |
| 关 键 词： | 蟆叶秋海棠 离体培养 愈伤组织 植株再生 正交设计 |
Study on Tissue Culture in Vitro of Begonia rex |
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| HE Yong,HE Ai-ping,YU Dan-qing,TIAN Zhi-hong. Study on Tissue Culture in Vitro of Begonia rex[J]. Hubei Agricultural Sciences, 2009, 48(8) | |
| Authors: | HE Yong HE Ai-ping YU Dan-qing TIAN Zhi-hong |
| Affiliation: | 1.College of Life Sciences;Yangtze University;Jingzhou 434025;Hubei;China;2.College of Life Sciences;Wuhan University;Wuhan 430072;China |
| Abstract: | The effects of different growth regulators,and different media on callus induction were studied with the orthogonal design L25(55) ,and young leaves of Begonia rex were used as explants. The results indicated that the types of medium had significant affection on callus induction of Begonia rex,the growth regulator had little influence on callus induction,and the callus could differentiate immediately on medium without growth regulator. The optimum medium supplemented with growth regulators for callus induct... |
| Keywords: | Begonia rex in vitro culture callus plant regeneration orthogonal design |
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