| 不同CpG-DNA序列对鸡ND HI影响的研究 |
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| 引用本文： | 周顺,张祯涛,王金宝,李俊,宋玉才,刘长龙,徐文,倪娇.不同CpG-DNA序列对鸡ND HI影响的研究[J].中国预防兽医学报,2005,27(5):394-396. |
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| 作者姓名： | 周顺 张祯涛 王金宝 李俊 宋玉才 刘长龙 徐文 倪娇 |
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| 作者单位： | 莱阳农学院,动物科技院,山东,莱阳,265200 |
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| 摘 要： | 将16种人工合成的CpG-DNA与新城疫Ⅳ弱毒苗(La Sota株)合用,免疫14日龄雏鸡,于免疫前及免疫后7、14、21天测鸡HI抗体效价,筛选出4种CpG-DNA短序并进行SPF鸡重复试验,结果表明:筛选的4种CpG短序(1.AACGTr AACGTr;2.AACGTC AACGTC;3.AGCGTC AGCGTC;4.AGCGCT AGCGCT)可显著提高鸡体的体液免疫水平.
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| 关 键 词： | 免疫佐剂 鸡 筛选 |
| 文章编号： | 1008-0589（2005）05-0394-03 |
| 收稿时间： | 2004-04-12 |
| 修稿时间： | 2004年4月12日 |
Selection of CpG-DNA with the most adjuvant effcet to chicken |
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| ZHOU Shun,ZHANG Zhen-tao,WANG Jin-bao,LI Jun,SONG Yu-cai,LIU Chang-long,XU Wen,NI Jiao.Selection of CpG-DNA with the most adjuvant effcet to chicken[J].Chinese Journal of Preventive Veterinary Medicine,2005,27(5):394-396. |
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| Authors: | ZHOU Shun ZHANG Zhen-tao WANG Jin-bao LI Jun SONG Yu-cai LIU Chang-long XU Wen NI Jiao |
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| Abstract: | Using the NDV model,16 CpG-DNA were injected into 14-day chicken with vaccine of NDV IV.HI assay was performed for detection the specific antibody to NDV in sera from inoculated chicken at 7 th,14 th and 21 stday after immunization.4 CpG-DNA were selected and redone to SPF chicken.In conclusion,these 4 CpG motifs(1.AACGTTAACGTT;2.AACGTC AACGTC;3.AGCGTC AGCGTC;4.AGCGCT AGCGCT) could efficiently enhance the humoral immune response. |
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| Keywords: | CpG-DNA |
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