牡丹愈伤组织的诱导及愈伤褐化抑制的研究

以凤丹为试验材料,比较不同外植体,不同基本培养基,不同激素组合对愈伤诱导率的影响;结果表明,以子叶和胚轴为外植体,在培养基1/2改良WPM+2,4-D1.0 mg·L-1+6-BA1.0 mg·L-1+水解酪蛋白500mg·L-1+L-谷氨酰胺150 mg·L-1+蔗糖30g·L-1+琼脂7g·L-1的培养基中诱导率最高,可达100%。同时研究了愈伤褐化抑制,结果表明,以1/2改良WPM为最佳基本培养基;PVP和植物凝胶的效果最好,褐化指数分别为31.11%与30.00%;黑暗、低温、较短的继代周期都能不同程度的减缓愈伤褐化。

The research on callus inducing and browning prevention of peony

This paper used Paeonia ostii var liishizhenii as experiment material.Compared the effect of different explants,basal mediums and combinations of hormones to callus inducing;the results indicated,to use cotyledon and radicel as explant,on the medium: 1/2 modified WPM+2,4-D 1.0 mg·L-1+ 6-BA1.0 mg·L-1+Casein hydrolysate 500 mg·L-1+L-glutamine 150 mg·L-1+sucrose 30g·L-1+ager 7 g·L-1,inducing rate reached to100%,In the research of the effect of basal medium,different concentration of inhibitor and culture condition to the browning,the result implied,browning was slighter in the basal medium 1/2 modified WPM;antioxidant and sorbent had remarkable browning prohibited effect,PVP and Phytagel took on the best browning prohibited effect in all of them.The browning index was 31.11% and 30.00%.Different culture conditions affected browning greatly,darkness,low-temperature and short subculture cycle can reduce browning in different degree.