Isolation, Identification of Differentially Expressed Sequence Tags in the Backfat Tissue from Meishan, large White and Meishan×Large White Cross Pigs |
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Authors: | LIU Yong-gang XIONG Yuan-Zhu DENG Chang-Yan |
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Abstract: | In order to detect the molecular mechanism of heterosis in pigs, the mRNA differential display technique was performed to investigate the differences of gene expression in the backfat tissue from Meishan,Large White and Meishan×Large White cross pigs.Nine 3'-end anchored primers in combination with ten 5'-end arbitrary primers were used to perform the differential display PCR and nearly 3 000 reproducible bands were examined .Fifteen expressed sepuence tags that were differentially expressed were isolated and then isdentifide through semi-quantitative RT-PCR.BLAST analysis revealed that the fifteen expressed sequence tags (ESTs) were ntt homologous to any of the known porcine genes or ESTs. These novel ESTs were then submitted to GenBank. |
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Keywords: | Pig mRNA differential display Semi-quantitative RT-PCR Expressed sequence tag |
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