利用基因工程改造瘤胃微生物调控瘤胃发酵研究进展

DNA分子的人工合成是基因工程技术的基础,改变反刍动物瘤胃微生物DNA的结构,能够使得动物表达出不同的蛋白质,反刍动物的生长模式也因此而发生不可预知的改变。正在应用的或处于研究阶段的构建瘤胃基因工程菌的方式主要有基因缺失技术、基因复制性重组技术和启动子的应用技术。其中基因缺失技术还包括转座子插入法和自杀性质粒的构建。目前影响基因工程技术成功率的最主要障碍就在于这种技术的低效率和不可预知性,一旦清除了这个障碍,基因工程技术将会在瘤胃微生物的改造上有更加广阔的发展空间。

Progress on Utilize Gene Engineering Reconstruct Rumen Bacteria to Regulate and Control Rumen Fermentation

The artificial recombination of DNA molecules is the basis of genetic engineering technique',the DNA of rumen bacteria is modified, hence different proteins are manufactured, and the development pattern of the ruminant animal is changed- often in an unpredictable way. The main methods used, or under investigation, for production of rumen gene engineering bacteria are:gene deletion technique, gene recombination technique and the application of promotor. The gene deletion technique include transposition bracketing method and the construction of suicide plasmid. Now the main technical barrier to success is the inefficiency and unpredictability of the genetic engineering technique. Once we wipe out the barrier, genetic engineering technique will has a more widely space in rumen bacteria.division.